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SUMO Fusions to Enhance Expression of Membrane Proteins

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
76338
Program Year/Program:
2005 / SBIR
Agency Tracking Number:
GM074316
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
LIFESENSORS, INC.
271 GREAT VALLEY PKY MALVERN, PA -
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2005
Title: SUMO Fusions to Enhance Expression of Membrane Proteins
Agency: HHS
Contract: 1R43GM074316-01
Award Amount: $206,308.00
 

Abstract:

DESCRIPTION (provided by applicant): Membrane proteins have crucial roles in many cellular and physiological processes. Understanding the structures and functions of these proteins is academically and commercially important. However, the lack of efficient methods for expressing membrane proteins has created a bottleneck. While one third of all proteins are membrane proteins, less than 100 membrane protein structures have been solved to the date. Among membrane proteins, G protein coupled receptors (GPCR) represent a large family of about 1000 proteins in human cells and are the most important drug targets. We propose the development of the SUMO-Fusion system to enhance expression and purification of a wide variety of membrane proteins in E. coli. The key features of the SUMO Fusion technology are: 1) attachment of the C-terminus of SUMO protein to the N terminus of a membrane protein to act as a chaperone, enhancing the qualitative and quantitative expression of the membrane protein; 2) introduction of N-terminal 6xHis-SUMO to act as a tag to allow rapid purification; 3) exploitation of SUMO proteases to provide efficient cleavage of SUMO fusions, thereby generating membrane proteins with native N-termini. The goal of this proposal is to develop and improve the SUMO fusion system for enhancement of expression and purification of model membrane proteins in E. coli.

Principal Investigator:

Xun Zuo
6106448845
ZUO@LIFESENSORS.COM

Business Contact:

Varsha Luthra
6106448845
Small Business Information at Submission:

Lifesensors, Inc.
Lifesensors, Inc. 271 Great Valley Pky Malvern, PA 19355

EIN/Tax ID: 232855125
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No