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HYBRID CELL FORMATION BY TARGETED ELECTROFUSION

Award Information

Agency:
National Science Foundation
Branch:
N/A
Award ID:
2655
Program Year/Program:
1988 / SBIR
Agency Tracking Number:
2655
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
LITRON LABORATORIES, LTD.
200 CANAL VIEW BLVD, STE 106 ROCHESTER, NY -
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1988
Title: HYBRID CELL FORMATION BY TARGETED ELECTROFUSION
Agency: NSF
Contract: N/A
Award Amount: $200,000.00
 

Abstract:

CELL FUSION PROVIDES BIOTECHNOLOGISTS WITH THE ABILITY TO GENERATE UNIQUE CELL TYPES BY MIXING THE GENETIC MATERIAL OFTWO PARENT CELLS. THE GENERATION OF IMMORTAL ANTIBODY PRODUCTING HYBRIDOMAS BY FUSING ANTIBODY PRODUCING SPLEEN CELLS WITH MYELOMA CELLS PROVIDES AN EXAMPLE OF THE UTILITY OF CELL FUSION TECHNOLOGY. HOWEVER, THE WIDELY USED CHEMICAL FUSION PROCEDURES, BASED ON POLYETHYLENEGLYCOL (PEG) ARE LABOR INTENSIVE, TIME CONSUMING AND COSTLY, AND THEY PROVIDE RELATIVELY LOW YIELDS OF THE DESIRED HYBRIDOMA.THE APPLICATION OF ELECTROFUSION (RATHER THAN CHEMICAL FUSION) IN CONJUNCTION WITH METHODS FOR TARGETING THE CELL POPULATIONS PRIOR TO FUSION, SHOULD PROVIDE AN IMPORTANT ADVANCE IN HYBRID CELL TECHNOLOGY. AS A RESULT OF THIS RESEARCH PROGRAM, WE WILL BE ABLE TO OBTAIN HIGHER YIELDS OFHYBRID CELLS, IN A SHORTER TIME AND AT A LOWER COST THAN PRESENT FUSION METHODS. THE HYBRIDOMA MODEL SYSTEM WILL PROVIDE THE TECHNOLOGICAL BASIS FOR EVENTUALLY TARGETING OTHER TYPES OF PLANT AND ANIMAL CELLS FOR ELECTROFUSION.

Principal Investigator:

Andrew M Tometsko Phd
Director Of Research
7162754008

Business Contact:

Small Business Information at Submission:

Litron Laboratorytories Ltd
1351 Mt Hope Ave #207 Rochester, NY 14620

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No