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AUTOMATION OF THE IN VITRO MICRONUCLEUS ASSAY

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
59826
Program Year/Program:
2002 / SBIR
Agency Tracking Number:
CA094493
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
LITRON LABORATORIES, LTD.
200 CANAL VIEW BLVD, STE 106 ROCHESTER, NY -
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2002
Title: AUTOMATION OF THE IN VITRO MICRONUCLEUS ASSAY
Agency: HHS
Contract: 1R43CA094493-01
Award Amount: $98,597.00
 

Abstract:

The in vitro nucleus test is increasingly being used as an alternative to the more costly and time consuming in vitro chromosome aberration assay as a screen for detecting a chemical's genotoxic potential (aneugenic or clastogenic activity which are associated with disorders such as Down's Syndrome, cancers, etc.) Micronucleus measurements are made using the time-consuming and tedious process of microscopic inspection. No definitive and widely-accepted automated approach has come into use. During Phase I, we will investigate the use of flow cytometry to enumerate micronuclei in mouse lymphoma L5178 cells. This cell line is used in a commonly performed forward mutation assays (i.e., thymidine kinase locus) required by regulatory agencies world-wide. These cells offer the potential to develop an assay where the cells are treated and subsequently split between micronucleus and mutation endpoints, thereby minimizing plasticware, test article, and time requirements. Studies will focus on identifying gating and fluorescent staining procedures to discriminate bodies and mitotic chromosomes from true micronucleus events. Long range, the automated scoring procedures developed can be extended to the analysis of micronuclei in human lymphocytes. Some of these applications may include: 1) assessing the genotoxicity of pharmaceuticals. 2) identifying the individuals hypersensitive in particular genotoxin, 3) evaluating compounds or diets protective against genetic damage and 4) measurement of DNA damage in a population following a radiation and/or chemical accident.

Principal Investigator:

Stephen D. Dertinger
5854420930
SDERTINGER@LITRONLABS.COM

Business Contact:

Carol Tometsko
7164420930
CAROLTOMET@AOL.COM
Small Business Information at Submission:

LITRON LABORATORIES, LTD.
LITRON LABORATORIES, LTD. 1351 MOUNT HOPE AVE ROCHESTER, NY 14620

EIN/Tax ID: 161080732
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No