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Assessing toxicity with an ex vivo erythropoiesis system

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
66444
Program Year/Program:
2003 / SBIR
Agency Tracking Number:
ES011915
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
LITRON LABORATORIES, LTD.
200 CANAL VIEW BLVD, STE 106 ROCHESTER, NY -
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2003
Title: Assessing toxicity with an ex vivo erythropoiesis system
Agency: HHS
Contract: 1R43ES011915-01A1
Award Amount: $100,000.00
 

Abstract:

DESCRIPTION (provided by applicant): Humans are exposed to toxic chemical and physical agents through a variety of sources. Of extreme concern are agents which can damage DNA, as genotoxicity is associated with cancer, atherosclerosis, and birth defects. The research effort proposed herein is aimed at developing murine ex vivo culture system that is capable of detecting agents which damage chromosomes through clastogenic and aneugenic activity. Currently, chemical-induced chromosome damage is evaluated by scoring chromosome abberations or micronucleus formation in cell lines or in laboratory rodents. By developing an ex vivo erythropoiesis system, reticulocytes can be generated in culture which serve as source material for micronucleus scoring. The primary cultures should provide more relevant toxicity information compared to immortilized cell lines. Furthermore, the feasibility of developing a flow cytometric scoring technique to enumerate micronuclei in ex vivo-derived reticulocytes is high, and overcomes artifacts associated with automated scoring of the standard in vitro assay. Relative to rodent tests, the proposed primary culture system will require few animals. To investigate the feasibility and utility of a genetic toxicity screening system based on ex vivo erythropoiesis, we will: 1. EVALUATE reticulocyte cell production by ex vivo culture(s). 2. MEASURE the frequency of micronuclei in ex vivo derived reticulocytes. 3. DETERMINE the responsiveness of the system to known clastogens and a model aneugen.

Principal Investigator:

Francis G. Murante
5854420930
FMURANTE@LITRONLABS.COM

Business Contact:


7164420930
Small Business Information at Submission:

LITRON LABORATORIES, LTD.
1351 MOUNT HOPE AVENUE SUITE 207 ROCHESTER, NY 14620

EIN/Tax ID: 161080732
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No