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CHEMILUMINESCENCE IMMUNOASSAY FOR INSULIN

Award Information

Agency:
Department of Health and Human Services 		Branch:
N/A 		Award ID:
7229 		Program Year/Program:
1987 / SBIR
Agency Tracking Number:
7229 		Solicitation Year:
N/A 		Solicitation Topic Code:
N/A 		Solicitation Number:
N/A
Small Business Information
London Diagnostics Inc
10300 Valley View Rd Suite 107 Eden Prairie, MN 55344
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1987
Title: CHEMILUMINESCENCE IMMUNOASSAY FOR INSULIN
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE AIM OF THIS PROJECT IS TO DEMONSTRATE THE FEASIBILITY OF AN IMMUNOASSAY FOR INSULIN, USING ACRIDINIUM ESTERS AS A CHEMILUMINESCENT LABEL. THIS WILL LEAD TO A TECHNIQUE FOR THE DETECTION OF POLYPEPTIDE HORMONES THAT IS FASTER AND MORE SENSITIVE, AND REMOVES THE NEED FOR (125)I-LABELED PEPTIDES OR ANTIBODIES. THE PROJECT WILL EXPLORE BOTH TRADITIONAL IMMUNOASSAYS IN WHICH THE INSULIN MOLECULE IS LABELED AND "SANDWICH"-TYPE ASSAYS IN WHICH INSULIN ANTIBODIES ARE USED. IN THE FIRST CASE, LABELED INSULIN WILL BE PURIFIED BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) AND DIRECTLY SUBSTITUTED AS THETRACER IN A RADIOIMMUNOASSAY (RIA) PROCEDURE. FOR THE IMMUNOMETRIC ASSAY, ANTIBODIES TO INSULIN WILL BE LABELED AND PURIFIED BY GEL FILTRATION CHROMATOGRAPHY. THE LABELED PEPTIDE AND ANTIBODY WILL BE COMPARED FOR STABILITY AGAINST A COMMERCIALLY AVAILABLE RIA USING (125)I-LABELED INSULIN. THE ASSAY WILL SERVE AS A MODEL FOROTHER PEPTIDE HORMONE ASSAYS THAT REQUIRE HIGH SENSITIVITY AND STABLE REAGENTS. THE PROJECT WILL INVOLVE LIMITED ASSAY DEVELOPMENT, COMPARISON WITH A COMMERCIAL RIA, AND A LIMITED TRIAL WITH PLASMA SAMPLES. THE MULTIMILLION-DOLLAR HORMONE RIA MARKET MIGHT INCREASE IFMORE STABLE, SAFER, CHEAPER, AND SIMPLER ASSAYS WERE AVAILABLE USING CHEMILUMINESCENCE DETECTION TECHNIQUES.

Principal Investigator:

Russell c hart phd
6129413812

Business Contact:

Small Business Information at Submission:

London Diagnostics Inc
10300 Valley View Rd Suite 107 Eden Prairie, MN 55344

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No