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Single Cell Genomics of Hyperthermophiles

Award Information

Agency:
Department of Energy
Branch:
N/A
Award ID:
57108
Program Year/Program:
2002 / SBIR
Agency Tracking Number:
70588S02-I
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
LUCIGEN CORPORATION
2905 PARMENTER ST. Middleton, WI 53562-2547
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2002
Title: Single Cell Genomics of Hyperthermophiles
Agency: DOE
Contract: DE-FG02-02ER83484
Award Amount: $100,000.00
 

Abstract:

70588 Modern methods of molecular analysis reveal an astonishing discrepancy between the diversity and numbers of organisms present in natural habitats and the relatively few capable of being cultured. Single Cell Genomics is a new initiative to develop the tools and methods for extracting cultivation-resistant genetic diversity in a form that is useful for whole genome sequence analysis and expression screening. This project will apply these tools to discover valuable hyperthermophilic enzymes and sequence information from a class of microorganisms especially difficult to grow in the lab. In Phase I, single microorganisms will be isolated from a diverse mixture using microscopy and a micromanipulator. Specific tasks include the isolation of a single molecule of intact genomic DNA, unbiased amplification with high fidelity, ligation to a new dual orientation zero-background cloning vector, and transformation into a new E. coli strain. A random genomic library from a single microbial cell adequate for 10X sequence coverage will be constructed. The long-term goals of this research are to develop a phylogenetically diverse set of genomic libraries from single cells of culture-resistant hyperthermophiles and screen them for commercially valuable enzymes. Commercial Applications and Other Benefits as described by the awardee: The technology could result in sales of reagent kits, a single cell genomic DNA cloning service, and hyperthermophilic genomic libraries. Enzyme activity screens for hyperthermophilic laccase and xylanase activities should provide environmentally friendly new reagents for the fiber processing and wood pulping industries.

Principal Investigator:

David A. Mead
6088319011
dmead@lucigen.com

Business Contact:

David A. Mead
70588
6088319011
dmead@lucigen.com
Small Business Information at Submission:

Lucigen Corp.
2120 W. Greenview Drive Middleton, WI 53562

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No