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ACCELERATED BAC LIBRARY CONSTRUCTION AND ANALYSIS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
60222
Program Year/Program:
2002 / SBIR
Agency Tracking Number:
HG002627
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
LUCIGEN CORPORATION
2905 PARMENTER ST. Middleton, WI 53562-2547
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2002
Title: ACCELERATED BAC LIBRARY CONSTRUCTION AND ANALYSIS
Agency: HHS
Contract: 1R43HG002627-01
Award Amount: $100,000.00
 

Abstract:

DESCRIPTION (provided by applicant): Large insert DNA libraries are essential for the molecular analysis of the human genome and other genomes important to our well-being. Comparative genomics reveals new information about metabolic pathways, cancer, horizontal gene transfer, evolution, protein families, and the genetic repertoire of numerous species. It has also greatly increased the demand for additional genomic BAC libraries. Construction of these libraries is time consuming, costly and challenging. The long-term objectives of this proposal are to facilitate cloning the current gaps in the human genome and reduce the time required for constructing large BAC libraries to a few weeks from several months. The quality of these libraries will surpass current standards for bias, randomness, fidelity, and low contamination. Specific aims include the development of new BAC cloning tools, strains, and methods that circumvent traditional bottlenecks and substantially improve the molecular cloning and transformation efficiency of high molecular weight genomic DNA. A new E. coli transformation system could improve large DNA uptake 100-fold and increase average insert size to above 300 kb. Other technical innovations include a simple method for randomly shearing high molecular weight DNA, a zero background cloning system, and a transcription-free cloning system that eliminates several forms of cloning bias. PROPOSED COMMERCIAL APPLICATIONS: Commercial products and services include: a new E. coli transformation system with exceptionally high DNA uptake efficiency, a zero background, transcription-free vector for unbiased cloning of recalcitrant genes and gneomes, a BAC library construction kt, a BAC DNA affinity purification kit, a vector pop-out to reduce re-sequencing of the plasmid backbone, and an economical BAC library construction service.

Principal Investigator:

David A. Mead
6088319011
DMEAD@LUCIGEN.COM

Business Contact:

David Mead
6088319011
DMEAD@LUCIGEN.COM
Small Business Information at Submission:

LUCIGEN CORPORATION
LUCIGEN CORPORATION 2120 W GREENVIEW DR MIDDLETON, WI 53562

EIN/Tax ID: 391923306
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No