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Ultrasensitive Chemiluminescent Enzyme-Linked Assays

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
25014
Program Year/Program:
1995 / SBIR
Agency Tracking Number:
25014
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
LUMIGEN, INC.
LUMIGEN, INC. 22900 W EIGHT MILE RD SOUTHFIELD, MI 48034 0430
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1995
Title: Ultrasensitive Chemiluminescent Enzyme-Linked Assays
Agency: HHS
Contract: 1 R43 DK47727-1,
Award Amount: $749,680.00
 

Abstract:

Our objective is to prove feasibility of ultrasensitive chemiluminescent assays for the 21 aminoacid polypeptide from HIV gp 120 by Western blotting and cystic fibrosis transmembrane regulator geneby Southern blotting. We have developed a proprietary enzyme-label chemiluminescent detection systemwith enhanced sensitivity in solid-phase detection formats. Proprietary substrates for the commonmarker enzyme alkaline phosphatase and enhancers provide excellent specificity, sensitivity and speedof detection. We will develop sets of chemiluminescent reagents to test feasibility of detection ofproteins and nucleic acids in the above assays. This research should prove the feasibility of a newgeneration of chemiluminescent reagents for enzyme detection and a novel method of chemiluminescentdetection of analytes of biomedical significance. The initial commercial application will be for HIV gp120 and cystic fibrosis transmembrane regulator gene, which will be developed in Phase Il.

Principal Investigator:

Hashem Akhavan-Tafti
3133515600

Business Contact:

Small Business Information at Submission:

Lumigen, Inc.
24485 W Ten Mile Road Southfield, MI 48034

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No