The work will lead to the development of disposable pipet tips for pre-existing adjustable micropipettors that will completely prevent carry-over contamination in polymerase chain reaction (pcr) gene amplification work. the tips will be similar to current tips in that they will be manufactured to… More

In the proposed research we will develop methodologies and products for mapping very large pieces of dna by determiningthe overlapping relationships between clones derived from a partial digest of the dna. the method will create the possibility of ordering end-to-end a large number of partial-digest… More

We will develop an inexpensive, disposable device for rapid acrylamide or agarose gel electrophoretic separation of dna, rna or protein which does not require running buffer or user performed steps other than sample application and connection of electrical leads. the device consists of a thin,… More

The Phase I work proposed is the development of devices and methods for sequencing of DNA by hybridization. Sequencing by hybridization (SBH) is a method in which a target DNA is sequenced by separate hybridization to a large number of short oligos [1]. We will develop a novel simultaneous multiple… More

A low cost, automated, benchtop instrument that can purify up to 24 samples of plasmid dna simultaneously in one hour is now available. the instrument uses a form of agarose gel electrophoresis to separate the plasmid dna and recovers it by electroelution into ~20 ul volume. the instrument has… More

There is a need for specific spatial and temporal cDNA libraries. A complete set of libraries acrostime periods will facilitate studies of gene function. Libraries representing different tissues andneeded to aid in the identification, localization, and characterization of the novel genes as they… More

50462-98-I Automated Purification of Blood or Bacterial Genomic DNA--MacConnell Research Corporation, 11339 Sorrento Valley Road, B, San Diego, CA 92121-1303; (619) 452-2603 Dr. William P. MacConnell, Principal Investigator Dr. William P. MacConnell, Business Official DOE Grant No.… More

Not Available Physical Sciences Inc. proposes to develop a system of variable color electrochromic (EC) devices and sensors for actively sensing the background environment of an object and adjusting its reflectivity in visible wavelengths to minimize its contrast with the environment. The… More

Not Available Theseus Logic has developed and demonstrated a proprietary new logic family, NULL Convention Logical (NCL), which integrates data transformation and control into a single expression thus producing inherently clockless, data driven, and effectively delay insensitive circuits and… More

DESCRIPTION (provided by applicant): In Phase II, we will develop a fully automatic, high-throughput, low-cost, multi-sample mRNA purification instrument and disposable sample cassettes. Unlike any other process currently available for mRNA purification, this instrument uses a… More

DESCRIPTION (provided by applicant): In Phase II, we will develop a fully automatic, high-throughput, low-cost, multi-sample mRNA purification instrument and disposable sample cassettes. Unlike any other process currently available for mRNA purification, this instrument uses a… More

DESCRIPTION (provided by applicant): In Phase I, we will develop a prototype of a simple, cost-effective device that allows bacterial growth in any type of 96 or 384 well microtiter plates or deep well blocks without the use of expensive equipment. The product will simplify… More

73169S03-I Production of proteins from molecularly cloned genes is currently a cumbersome and difficult process requiring gene cloning, vector construction, optimization of expression, and purification from the host cell source. In addition, in vivo protein expression is often compromised by cell… More

DESCRIPTION (provided by applicant): In Phase II, we will develop, and test a simple, cost-effective device that allows bacterial growth in any type of 96 or 384 well microtiter plates or deep well blocks without the use of expensive equipment. The product simplifies bacterial growth in tightly… More

DESCRIPTION (provided by applicant): In Phase II, we will develop, and test a simple, cost-effective device that allows bacterial growth in any type of 96 or 384 well microtiter plates or deep well blocks without the use of expensive equipment. The product simplifies bacterial growth in tightly… More

DESCRIPTION (provided by applicant): In this proposal, we will describe a new method and instrument for real-time, target-specific isothermal amplification and DNA quantitation that does not rely on the polymerase chain reaction and does not require expensive fluorescent labeled oligo primers. This… More

73169-The production of proteins from cloned DNA sequences is an important process for functional genomic studies and structural analysis, as well as many other research applications, including pharmaceutical drug discovery. This project will develop in vitro protein synthesis reagents and… More

DESCRIPTION (provided by applicant): The advent of real-time PCR instrumentation and reagents allows rapid and highly specific quantification of genes and messenger RNAs (1-3). Real-time PCR gene amplification has significantly changed the way microbial pathogens are detected, identified and… More

DESCRIPTION (provided by applicant): We will develop a novel method for site-specific cleavage of double-stranded DNA for in vitro and in vivo applications. This method will create a new class of DNA restriction enzymes that can be designed by the researcher to specifically recognize a 20 base pair… More

DESCRIPTION (provided by applicant): Protein production from cloned DNA sequences has become increasingly important in the post-genomic era. The availability of sequences from whole bacterial genomes, cDNAs, and tens of thousands of predicted mammalian genes have created the need to produce proteins… More

DESCRIPTION (provided by applicant): The aim of this work is to develop a low cost, automated, multi-sample device and method that will allow for rapid and efficient purification of genomic DNA from a wide variety of tissue, cell and bacterial samples. Th is process will be optimized to purify DNA… More

DESCRIPTION (provided by applicant): The aim of the work is to develop a low cost, automated, multi-sample device and method that will allow rapid and efficient purification of genomic DNA from a wide variety of tissue, cell, and bacterial samples. The p rocess will be optimized to work with… More

DESCRIPTION (provided by applicant): This work will create innovative products that allow rapid, low cost, automated, multi- sample genomic DNA purification from many sample types and concentrations. The novel technology that we will further develop and validate in Phase I, uses disposable cassette… More

DESCRIPTION (provided by applicant): This work will create innovative products that allow rapid, low cost, automated, genomic DNA purification from several sample types and concentrations. This novel technology uses a disposable cassette device and low cost, reusable processing cradle to… More

DESCRIPTION (provided by applicant): This research involves the development a disposable purification cassette and bench-top electrophoretic processing instrument that will purify one milligram or more of plasmid DNA from recombinant culture in 60 minutes.The purification method to be employed in… More

DESCRIPTION (provided by applicant): Phase I is the development of a large-array oligonucleotide synthesis instrument that will greatly decrease the cost and simplify construction of mega base-size double-stranded DNA segments. This novel technology will use less than 1/40th of the synthesis… More