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MODIFIED SURFACES FOR CHEMICALLY DEFINED CELL CULTURE

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
14057
Program Year/Program:
1990 / SBIR
Agency Tracking Number:
14057
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
MATTEK CORPORATION
200 HOMER AVE ASHLAND, MA -
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1990
Title: MODIFIED SURFACES FOR CHEMICALLY DEFINED CELL CULTURE
Agency: HHS
Contract: N/A
Award Amount: $15,760.00
 

Abstract:

THE PRIMARY GOAL OF THIS RESEARCH IS THE DEVELOPMENT OF NOVEL PLASTICS ON WHICH TO CULTURE CELLS IN SERUM-FREE MEDIA. CURRENTLY, BIOLOGICAL ATTACHMENT AIDS MUST BE USED FOR ANCHORAGE-DEPENDENT CELLS TO ATTACH AND GROW NORMALLY INTHE ABSENCE OF SERUM. LOW-TEMPERATURE GAS PLASMA TECHNIQUES WILL BE EMPLOYED TO MODIFY THE SURFACES OF POLYSTYRENE CULTUREWARE. SUCCESS WILL BE DETERMINED BY THE ABILITY OF THE MODIFIED SURFACES TO FOSTER ATTACHMENT AND GROWTH OF ANCHORAGE-DEPENDENT CELLSIN CHEMICALLY DEFINED MEDIA. IN ADDITION, A SECONDARY GOAL WOULD BE ACHIEVED IF A SURFACE WAS DEVELOPED THAT ENHANCED CELL ATTACHMENT AND GROWTH IN VERY LOW SERUM CONCENTRATIONS. TWO REPRESENTATIVE CELL TYPES WILL BE USED: PRIMARY CHICK EMBRYO FIBROBLASTS AND CHINESE HAMSTER OVARY CELLS. ALL CELLS WILL BE CULTURED IN THEIR RECOMMENDED MEDIUM WITH NORMAL SERUM SUPPLEMENTS AS A CONTROL, IN A MEDIUM WITH LOW SERUM, AND IN A NUMBER OF CHEMICALLY DEFINED MEDIA. COMPARISONS OF ATTACHMENT AND GROWTH ON PLASMA-TREATED SURFACES AND ON COLLAGEN WILL BE MADE FOR ALL CULTURE CONDTIONS.

Principal Investigator:

Mitchell Klausner
5088816771

Business Contact:

Small Business Information at Submission:

Mattek Corp
200 Homer Ave Ashland, MA 01721

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No