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In Vitro Assay For Chemical Allergens

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
70638
Program Year/Program:
2004 / SBIR
Agency Tracking Number:
CA106137
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
MATTEK CORPORATION
200 HOMER AVE ASHLAND, MA -
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2004
Title: In Vitro Assay For Chemical Allergens
Agency: HHS
Contract: 1R43CA106137-01
Award Amount: $206,494.00
 

Abstract:

DESCRIPTION (provided by applicant): Allergic contact dermatitis (ACD) is one of the most common and costly health problems in industrialized countries. Currently more than 100,000 chemicals are in commercial use and another 2,000 new chemicals and numerous new-product formulations are tested or marketed every year. Epicutaneous exposure to these chemicals could induce undesirable immunological skin reactions such as ACD. Although various in vivo animal models are used to evaluate potential allergenicity, these tests are subjective, time-consuming, and expensive. Therefore, an economic, in vitro predictive assay system that utilizes cells of human origin would find broad commercial utility. Various researchers have shown the critical role of dendritic cells (DC) in the initial steps of ACD such as priming naive T-cells (TC) to allergens. Here we propose an in vitro assay that utilizes a subset of dendritic cells to induce TC proliferation in predictive tests for ACD as a replacement for the local lymph node assay (LLNA). Versus the LLNA, the proposed assay is advantageous in that it utilizes cells of human origin and it eliminates the use of radioisotopes to model the primary and secondary immune responses involved in ACD. This research will develop a highly sensitive, nonanimal assay, which will be a cost-effective and accurate means of identifying contact allergens. Allergic contact dermatitis, in vitro allergenicity testing, plasmacytoid DC, myeloid DC.

Principal Investigator:

Seyoum Ayehunie
5088816771
SAYEHUNIE@MATTEK.COM

Business Contact:

John Sheasgreen
5088816771
JSHEASGREEN@MATTEK.COM
Small Business Information at Submission:

MATTEK CORPORATION
MATTEK CORPORATION 200 HOMER AVE ASHLAND, MA 01721

EIN/Tax ID: 042877744
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No