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Reconstructed Human Oral Tissues

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
66304
Program Year/Program:
2003 / SBIR
Agency Tracking Number:
DE013277
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
MATTEK CORPORATION
200 HOMER AVE ASHLAND, MA -
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2003
Title: Reconstructed Human Oral Tissues
Agency: HHS
Contract: 2R44DE013277-02
Award Amount: $0.00
 

Abstract:

DESCRIPTION (provided by applicant): Phase I research adapted primary culture methods to obtain a reliable source of normal human buccal epithelial (NHBE) cells. Tissue culture methods were developed to induce the NHBE cells to differentiate into a three-dimensional organotypic tissue. Characterization showed that the tissue reproduced many of the histological, protein expression, and barrier function properties of native buccal tissue. Initial experiments investigated use of the NHBE tissue for predicting oral irritation and transbuccal drug delivery rates. Finally, an economic evaluation of costs involved in tissue production showed that an NHBE product appears to be commercially feasible. The Phase II work plan will expand upon the results of Phase I to further develop the NHBE and other related, and more complex, oral mucosal models. NHBE culture conditions will be optimized and conditions to produce a gingival phenotype and incorporate lamina propria will be determined. Protocols to predict oral irritation will be determined and use of the tissue for transbuccal drug delivery will be studied in a more comprehensive manner. Finally, studies addressing basic oral biology phenomena, namely anti-microbial peptide regulation and HIV infection, will be performed.

Principal Investigator:

Mitchell Klausner
5088816771
MKLAUSNER@MATTEK.COM

Business Contact:

John Sheasgreen
5088816771
JSHEASGREEN@MATTEK.COM
Small Business Information at Submission:

MATTEK CORPORATION
MATTEK CORPORATION 200 HOMER AVE ASHLAND, MA 01721

EIN/Tax ID: 042877744
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No