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Phototoxicity using the Chorioallantoic Membrane

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
54477
Program Year/Program:
2001 / SBIR
Agency Tracking Number:
1R43ES010805-01A1
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Mb Research Laboratories, Inc.
1765 WENTZ ROAD PO BOX 178 SPINNERSTOWN, PA 18968-
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2001
Title: Phototoxicity using the Chorioallantoic Membrane
Agency: HHS
Contract: N/A
Award Amount: $100,000.00
 

Abstract:

DESCRIPTION (Applicant's Abstract): This proposal, "Phototoxicity Screen using the Chorioallantoic Membrane" (PhotoCAM) is an alternative phototoxicity screening test to identify chemical mediators and substances with phototoxic properties, which is in demand by the pharmaceutical, chemical, and consumer product industries. The chorioallantoic membrane (CAM) of the fertile chicken egg has already shown to be a useful, sensitive model for detecting irritants. The purpose of this proposal is to demonstrate the feasibility of employing the CAM assay, as well as endpoints of PGE2 and cytokine production by the epidermal cells comprising the tissue, as test systems to identify potential phototoxins or photoirritants. There have only been two papers published in the literature using this model, thus this is a relatively novel application of this model system. The use of an intact tissue has several advantages over existing cell culture-based phototoxicity screening assays. These include the presence of multiple cell types found in the skin, and a readily accessible circulatory system that is in contact with metabolically functional hepatic tissue. The PhotoCAM Assay described herein will provide a sensitive test system while (1) complying with the Animal Welfare Act (AWA) by directly addressing the 3Rs, by reducing animal usage; (2) increasing the discriminating power and quality of data generated when compared to cell culture methods; and (3) greatly reducing the costs associated with the use of animals for phototoxicity screening studies.

Principal Investigator:

Daniel R. Cerven

Business Contact:


2155364110
DEGEORGE@MBRESEARCH.COM
Small Business Information at Submission:

MB RESEARCH LABORATORIES, INC.
BOX 178, 1756 WENTZ RD SPINNERSTOWN, PA 18968

EIN/Tax ID: 232953302
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No