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HYPEREXPRESSION SYSTEM FOR RECOMBINANT BCG VACCINES

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
18947
Program Year/Program:
1992 / SBIR
Agency Tracking Number:
18947
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
MEDIMMUNE, INC.
35 WEST WATKINS MILL RD Gaithersburg, MD 20878
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1992
Title: HYPEREXPRESSION SYSTEM FOR RECOMBINANT BCG VACCINES
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

BCG, A LIVE ATTENUATED STRAIN OF MYCOBACTERIUM BOVIS, OFFERS A NUMBER A DISTINCT ADVANTAGES AS A LIVE RECOMBINANT VACCINE VEHICLE. THESE ADVANTAGES INCLUDE POTENT ADJUVANT ACTIVITY AND AHISTORY OF SAFE USE IN HUMANS AS A TUBERCULOSIS VACCINE. MAJOR GENETIC OBSTACLES IMPEDING THE DEVELOPMENT OF BCG AS A VACCINE VEHICLE HAVE NOW BEEN LARGELY OVERCOME WITH THE DEVELOPMENT OF ESCHERICHIA COLI/MYCOBACTERIAL SHUTTLE VECTORS. RECENT IMMUNOGENICITY STUDIES WITH SEVERAL RECOMBINANT BCG-BASED VACCINES ARE PROMISING AND SUGGEST THAT HIGH-LEVEL EXPRESSION OF TARGET ANTIGEN MAY CONTRIBUTE TO ITS ANTIGEN IMMUNODOMINANCE IN THE COMPLEX BACKGROUND OF MYCOBACTERIAL PROTEINS. SINCE IT HAS BEEN OBSERVED THAT CONSTITUTIVE HIGH-LEVEL EXPRESSION OF SOME FOREIGN ANTIGENS IN BCG IS DELETERIOUS TO THE BCG HOST STRAIN, WE WILL DEVELOP A BCG VECTOR/HOST SYSTEM INCORPORATING BCG STRESS PROMOTERS WITH TRANSCRIPTIONAL COMPONENTS FROM BACTERIOPHAGE T7 (T7 RNA POLYMERASE) AND THE E. COLI LACTOSE OPERON (LAC REPRESSOR AND OPERATOR TO REGULATE THE HIGH-LEVEL SYNTHESIS OF RECOMBINANT ANTIGENS IN BCG. IN ADDITION TO PROVIDINGAN INEXPENSIVE SOURCE OF ANTIGEN IN AN IMMUNOGENIC FORMAT WITHOUT ANTIGEN PURIFICATION, IT IS ALSO POSSIBLE THAT THE BCG/T7 SYSTEM COULD PROVIDE AN ALTERNATIVE TO LIVE RECOMBINANT BCG VACCINES WHICH MIGHT NOT BE SUITABLE IN IMMUNOCOMPROMISED INDIVIDUALS.

Principal Investigator:

Charles K Stover
3014170770

Business Contact:

Small Business Information at Submission:

Medimmune Inc
35 West Watkins Mill Road Gaithersburg, MD 20878

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No