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Efficient Coding Strategies for DNA Sequencing

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
22100
Program Year/Program:
1993 / SBIR
Agency Tracking Number:
22100
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Megabase Research Products
4711 Huntington Ave., Suite 2W Lincoln, NE 68504
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Woman-Owned: Yes
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1993
Title: Efficient Coding Strategies for DNA Sequencing
Agency: HHS
Contract: N/A
Award Amount: $49,951.00
 

Abstract:

Enzymatic dideoxy sequencing can be streamlined using algebraic coding strategies from communications theory. Using coding methods similar to those applied to telecommunications and digital electronics, the speed and efficiency of DNA sequencing can be substantially improved. One DNA template can be sequenced using three mixed dideoxy reactions and data channels; two templates can be read simultaneously in five channels. Having demonstrated that such 3-channel coding and 5:2 source code compression can be applied to 35-S dideoxy sequencing, experiments are proposed to improve the speed and efficiency of (i) multiplex DNA sequencing, (ii) PCR-based sequencing, (iii) automated multi-fluor sequencing, and (iv) automated multi-channel/single fluor sequencing. Proposed experiments are a cost-effective means of significantly improving a variety of existing manual and automated DNA sequencing techniques by 33-60%; further improvements are possible.

Principal Investigator:

Robert M. Nelson
4024660680

Business Contact:

Small Business Information at Submission:

Megabase Research Products
4129 Holdrege #2 Lincoln, NE 68503

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No