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Commercial Scale Production of Cvi Endonucleases

Award Information

Department of Health and Human Services
Award ID:
Program Year/Program:
1995 / SBIR
Agency Tracking Number:
Solicitation Year:
Solicitation Topic Code:
Solicitation Number:
Small Business Information
Megabase Research Products
4711 Huntington Ave., Suite 2W Lincoln, NE 68504
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Woman-Owned: Yes
Minority-Owned: No
HUBZone-Owned: No
Phase 2
Fiscal Year: 1995
Title: Commercial Scale Production of Cvi Endonucleases
Agency: HHS
Contract: N/A
Award Amount: $454,680.00


Chlorella virus (Cvi) restriction nucleases are a large, untapped source of gene mapping tools that have considerable commercial potential. Unlike bacterial restriction endonucleases, which recognize 4 to 8 base pair (bp) DNA target sequences, Cvi endonucleases define unique 2 to 4 bp cleavage sites and lend themselves to a variety of novel fine structure gene mapping applications. However, at the present time technology transfer from laboratory-scale enzyme identification and purification has not proceeded to large-scale commercial production. We will begin the development of large-scale production of three chlorella virus DNA restriction endonucleases (R.CviJI cleaves PuG/CPY, R.CviRI cleaves TG/CA, and R.CviAII cleaves C/ATG) for which there is a clearcut demand and cannot be isolated from bacteria. Two independent approaches toward large-scale (> 100,000 enzyme units) commercial production will be examined. (i) Increased production will be sought from virus-infected algae. (ii) The DNA restriction endonuclease gene will be cloned and overexpressed in E. coli. These studies will serve as an instructive model for the commercial scale production of a major new class of gene mapping enzymes.

Principal Investigator:

Robert M. Nelson

Business Contact:

Small Business Information at Submission:

Megabase Research Products
4129 Holdrege Lincoln, NE 68503

Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No