This proposal describes the design and construction of a prototype instrument for cell selection and sorting. this device can be applied to cells in culture which are attached to a solid support and maintained under favorable conditions for growth. this environment will be sustained within a novel… More

Mass spectrometry (ms) combined with gas chromatography, (gc) is the most powerful technique available for identifying bioactive compounds and for the selective detection of drug metabolites. recent improvements in gc resolution have surpassed increases in the speed and sensitivity of ms, thus… More

Confocal microscopy is one of the most significant advances in optical microscopy. its ability to virtually eliminate out-of-focus blurring permits optical sectioning of cells and thick transparent specimens. this technique is rapidly being adopted for the three- dimensional visualization of… More

We will develop a new generation confocal microscope uniquely capable of ultraviolet (UV) as well as visible illumination and video-rate imaging. In Phase I, experiments will be conducted to determine the components and dimensions needed to correct for the longitudinal and lateral chromatic errors… More

Optical microscopy is an important tool for investigations of biological structure and function inFar-field optics are restricted in resolution, however, to a physically defined limit of 200 nm. Invsubcellular organization and structure below this limit have required electron microscopy, which… More