Loci for use in routine screening in the l5178y mouse lymphoour aim is to determine whether mutation at the tk locus in l5178y mouse lymphoma cells is representative of mutation throughout the entire mammalian genome. mutation induction will be compared at three loci in l5178y cells and two loci in… More

It is the objective of this proposed phase i effort to develop a rapid, sensitive assay for detection of cytomegalovirus in the blood, saliva, or urine of human subjects using the capelisa system developed at microbiological associates, inc. this system places the antigen and antibody into an… More

The proposed contract will examine the abilities of various cell systems to detect oncogenes or proto-oncogenes. the primary objective during phase i will be to characterize thesensitivity of a variety of cell culture systems both with respect to transfectability (ability to take up and express… More

The overall goal of this study is the development of two in vitro transformation assays for the evaluation of chemo- preventive agents. the two systems utilize primary syrian hamster embryo cells and retroviral-infected fischer rat embryo cells (a continuous cell culture). both cell types are… More

Microbiologically defined laboratory animals are required for sophisticated biomedical research. serological procedures are the most feasible and economical means to monitor their health status. tyzzer's disease is one of themore insidious diseases of laboratory rodents and rabbits for which a… More

Microbiologically-defined mice and rats are required for sophisticated biomedical research. serological procedures are the most feasible and economical means to monitor the health status of laboratory animals. a major problem ariseswhen the results of various test-kits and in-house testing… More

Two distinct, virally induced infections in nonhuman primates that cause substantial morbidity and mortality and have clinical relevance to the human aids virus have been identified recently. the first, designated saids, is caused by a variety of closely related type d viruses. the second is induced… More

The purpose of this project is to improve the hamster embryo cell clonal transformation assay to increase the morphological transformation rate and introduce an additional endpoint, colony formation in soft agarose. the methodology described introduces a selective step which entails suspension of… More

The purpose of this research is to establish a rapid, inexpensive, in vitro system for studying the cytotoxicity and genotoxicity of particulates potentially hazardous to the respiratory system. short-term tests currently available to the research community were designed and optimized using soluble… More

A major challenge to federal and state regulatory agencies is the performance of hazard and risk assessments on chemicals aready in the environment and on new chemicals whose release is proposed. the defenitive experimental means for determining a material's carcinogenic potential remains the rodent… More