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ADAPTATION OF ELISA/IFAT FOR SERODIAGNOSIS OF TYZZER'S DISEASE

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
7384
Program Year/Program:
1987 / SBIR
Agency Tracking Number:
7384
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Microbiological Associates Inc
9900 Blackwell Road Rockville, MD 20850
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1987
Title: ADAPTATION OF ELISA/IFAT FOR SERODIAGNOSIS OF TYZZER'S DISEASE
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

MICROBIOLOGICALLY DEFINED LABORATORY ANIMALS ARE REQUIRED FOR SOPHISTICATED BIOMEDICAL RESEARCH. SEROLOGICAL PROCEDURES ARE THE MOST FEASIBLE AND ECONOMICAL MEANS TO MONITOR THEIR HEALTH STATUS. TYZZER'S DISEASE IS ONE OF THEMORE INSIDIOUS DISEASES OF LABORATORY RODENTS AND RABBITS FOR WHICH A SEROLOGIC TEST PROCEDURE IS NOT AVAILABLE COMMERCIALLY. THE ELUSIVE FASTIDIOUS ETIOLOGIC AGENT, BACILLUS PILIFORMIS (TYZZER), IS A GRAM-NEGATIVE, SPORE-FORMING, FLAGELLATED, OBLIGATE INTRACELLULAR PARASITE THAT REMAINS UNCLASSIFIED. TYPICAL HISTOPATHOLOGY FORMS THE BASIS FOR DIAGNOSIS IN 18 ANIMAL SPECIES. IMMUNOSUPPRESSION PRECIPITATES CLINICAL DISEASE, AND TRANSMISSION OCCURS VERTICALLY AS WELL AS HORIZONTALLY. SEROLOGICAL SURVEYS IN JAPAN AND EUROPE INDICATE THAT INAPPARENT INFECTIONS ARE COMMON IN CESAREAN-DERIVED, BARRIER-MAINTAINED COLONIES OF RATS. MICROBIOLOGICAL ASSOCIATES, INC. PROPOSES TO PREPARE SEED POOLS OF THREE STRAINS OF B. PILIFORMIS (PROPAGATED IN EMBRYONATED EGGS AND 3T3 CELL CULTURES), PREPARE ANTISERA TO EACH STRAIN IN MICE, ADAPT THE ELISA AND IFAT TECHNIQUES FOR SERODIAGNOSIS, DETERMINE THE DEGREE OF ANTIGENIC RELATEDNESS BETWEEN STRAINS, AND PROVIDE SERODIAGNOSTIC SERVICES TO THE BIOMEDICAL RESEARCH COMMUNITY AT REASONABLE COST.

Principal Investigator:

Robert l peters phd
3017381000

Business Contact:

Small Business Information at Submission:

Microbiological Associates Inc
9900 Blackwell Road Rockville, MD 20850

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No