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AN IN VITRO ASSAY FOR TOXIC PARTICULATES

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
11225
Program Year/Program:
1989 / SBIR
Agency Tracking Number:
11225
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Microbiological Associates Inc
9900 Blackwell Road Rockville, MD 20850
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1989
Title: AN IN VITRO ASSAY FOR TOXIC PARTICULATES
Agency: HHS
Contract: N/A
Award Amount: $48,132.00
 

Abstract:

THE PURPOSE OF THIS RESEARCH IS TO ESTABLISH A RAPID, INEXPENSIVE, IN VITRO SYSTEM FOR STUDYING THE CYTOTOXICITY AND GENOTOXICITY OF PARTICULATES POTENTIALLY HAZARDOUS TO THE RESPIRATORY SYSTEM. SHORT-TERM TESTS CURRENTLY AVAILABLE TO THE RESEARCH COMMUNITY WERE DESIGNED AND OPTIMIZED USING SOLUBLE TEST MATERIALS AND HAVE BEEN ADAPTED TO THE STUDY OF PARTICULATES WITH LIMITED PHAGOCYTICCAPACITY OF THE TARGET CELLS. THE ASSAY SYSTEM TO BE DEVELOPED WILL USE A FUNCTIONAL MOUSE MACROPHAGE CELL LINE. THIS CELL LINE, RAW264.7, IS AVIDLY PHAGOCYTIC, CAN UNDERGO A RESPIRATORY BURST, AND GROWS READILY IN CULTURE. THIS LATTER FEATURE WILL ALLOW BOTH CYTOTOXICITY AND GENOTOXICITY ENDPOINTS TO BE MEASURED. THE PHASE I GOALS ARE TO: (1) ESTABLISH THE OPTIMAL CONDITIONS FOR A CYTOTOXICITY ASSAY, (2) VALIDATE THE CYTOTOXICITY ASSAY WITH KNOWN TOXIC PARTICULATES, (3) ESTABLISH THE ASSAY CONDITIONS FOR AN HGPRT-BASED FORWARD MUTATION ASSAY, AND (4) ASSAY SEVERAL KNOWN CLASSES OF MUTAGENIC PARTICULATES.

Principal Investigator:


0

Business Contact:

Small Business Information at Submission:

Microbiological Associates Inc
9900 Blackwell Rd Rockville, MD 20850

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No