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MEASUREMENT OF FAST ENZYME KINETICS VIA DROPLET MERGING

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
16619
Program Year/Program:
1991 / SBIR
Agency Tracking Number:
16619
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
MicroFab Technologies Inc
1104 Summit Avenue Suite 110 Plano, TX -
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1991
Title: MEASUREMENT OF FAST ENZYME KINETICS VIA DROPLET MERGING
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

THE USE OF HIGH SPEED (1-10M/S) 25-200FM DIAMETER DROPLETS MERGING IN FLIGHT WILL BE USED TO MEASURE ENZYME KINETICS IN THE 50FS-1MS RANGE. TWO EXTREMELY REPEATABLE DROPLET STREAMS ARE PRODUCED USING TECHNIQUES DEVELOPED FOR INK-JET PRINTING SYSTEMS. THE TWO DROPLET STREAMS MERGE AND A REACTION WILL TAKE PLACE ALMOST INSTANTANEOUSLY. THE ENZYME REACTION CAUSES CHANGES IN ABSORPTION, FLUORESCENCE, OR CHEMILUMINESCENCE, DEPENDING ON THE ENZYME AND SUBSTRATE USED IN THE REACTION. IN PHASE I OF THIS STUDY, CHEMILUMINESCENT REACTIONS WILL BE EVALUATED. THE PHOTONS EMITTED ARE DETECTED BY A PHOTOMULTIPLIER TUBE FOCUSED ON A REGION OF THE MERGED DROPLET STREAM. THE REGION CORRESPONDS TO THE LENGTH A DROPLET WOULD TRAVEL IN 1MS. THE DETECTOR IS GATED TO MEASURE EMISSIONS FOR @FS-1MS. (WITH 1FS RESOLUTION) AND THE GATE IS SYNCHRONIZED WITH THE DROPLET CREATION FREQUENCY, ALLOWING THE EMISSIONS FROM THOUSANDS OF DROPS TO BE INTEGRATED. THE SMALL DIAMETER OF THE DROPLETS INSURES RAPID MIXING. THE REPEATABILITY OF DROPLET FORMATION INHERENT WITH INK-JET TECHNOLOGY ALLOWS PHOTONS TO BE GATHERED OVER LONG TIME PERIODS, THUS INCREASING THE SENSITIVITY OF THE MEASUREMENT.

Principal Investigator:

Wallace, David B
Principal Investigator
2145788076

Business Contact:

1 r43 gm44461-01a1
Small Business Information at Submission:

Microfab Technologies, Inc
1104 Summit Avenue Plano, TX 75074

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No