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Ink-jet Matrix Coating System for MALDI MS Imaging

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
85965
Program Year/Program:
2007 / SBIR
Agency Tracking Number:
RR024066
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
MicroFab Technologies Inc
1104 Summit Avenue Suite 110 Plano, TX -
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2007
Title: Ink-jet Matrix Coating System for MALDI MS Imaging
Agency: HHS
Contract: 1R43RR024066-01
Award Amount: $135,098.00
 

Abstract:

DESCRIPTION (provided by applicant): In this project, MicroFab will build a prototype system capable of depositing matrix solutions onto the tissue sections with high resolution using picoliter volumes (nanoliter volumes via the accretion of drops) for the purposes of MALDI MS imaging. The system will be very flexible in terms of amount of matrix deposited per spot, distribution of the matrix on the tissue sample, and the matrix spot pitch and placement. The system will provide capability for overprinting a dditional matrix solution and/or solvents and surfactants onto previously deposited matrix spots to improve analyte extraction from the protein sample. We will optimize the formulation of the matrix solutions to achieve the most reliable microdispensing, w hile maintaining functionality for the imaging process. The actual coating process will be verified by deposition onto tissue sections with evaluation by MALDI-TOF MS. The matrix coating platform is an automated tool for the precise microdeposition of matr ix solutions onto tissue samples for MALDI MS imaging. The array of discrete matrix spots significantly reduces the lateral migration of protein on the surface of the tissue section, while increasing the analyte extraction per area, resulting in a greater resolved protein image.

Principal Investigator:

Patrick W. Cooley
9725788076
PATRICK.COOLEY@MICROFAB.COM

Business Contact:

Patrick W. Cooley
david.wallace@microfab.com
Small Business Information at Submission:

MICROFAB TECHNOLOGIES, INC.
MICROFAB TECHNOLOGIES, INC. 1104 SUMMIT AVE, STE 110 PLANO, TX 75074

EIN/Tax ID: 751968264
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No