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BACULOVIRUS RECOMBINANTS THAT EXPRESS HEPATITIS B VIRUS SURFACE AN TIGEN:…

Award Information

Agency:
Department of Defense
Branch:
Army
Award ID:
1956
Program Year/Program:
1987 / SBIR
Agency Tracking Number:
1956
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Microgenesys Inc.
400 Frontage Rd W Haven, CT 06516
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1987
Title: BACULOVIRUS RECOMBINANTS THAT EXPRESS HEPATITIS B VIRUS SURFACE AN TIGEN: DEMONSTRATION FOR PRODUCTION OF SUBUNIT VACCINES
Agency / Branch: DOD / ARMY
Contract: N/A
Award Amount: $548,054.00
 

Abstract:

RECENTLY IT HAS BEEN DEMONSTRATED THAT BACULOVIRUSES CAN BE USED AS HIGH EFFICIENCY EUKARYOTIC CLONING AND EXPRESSION VECTORS FOR THE PRODUCTION OF FOREIGN PROTEINS. WE PROPOSE TO DEMONSTRATE THE FEASIBILITY AND BENEFITS OF USING THE BACULOVIRUS EXPRESSION SYSTEM FOR PRODUCTION OF SUBUNIT VACCINES. THIS DEMONSTRATION WILL BE EXEMPLIFIED BY THE EXPRESSION OF THE HBSAG-GENE IN INSECT CELLS INFECTED WITH RECOMBINANT BACULOVIRUS. SPECIAL ATTENTION WILL BE PAID TO THE TIME INVOLVED AND COST-EFFECTIVENESS OF OBTAINING THE FINAL PRODUCT. THIS DEMONSTRATION WILL INVOLVE THE FOLLOWING TASKS: CONSTRUCTION OF A RECOMBINANT BACULOVIRUS WHICH CONTAINS THE CODING SEQUENCE FOR HEPATITIS B VIRUS SURFACE ANTIGEN (HGSAG) UNDER THE CONTROL OF A BACULOVIRUS POLYHEDRIN-GENE PROMOTER. BIOCHEMICAL AND IMMUNOLOGICAL CHARACTERIZATION OF THE HBSAG GENERATED BY RECOMBINANT VIRUS IN INFECTED INVERTEBRATE TISSUE CULTURE CELLS AND CELL MEDIA. ANALYSIS OF STABILITY OF RECOMBINANT VIRUS DURING SEVERAL VIRUS GENEREATIONS. PILOT STUDY OF SCALED UP PRODUCTION IN SPINNER FLASKS.

Principal Investigator:

Dr Mark A Cochran
2039323203

Business Contact:

Small Business Information at Submission:

Microgenesys Inc.
400 Frontage Rd W Haven, CT 06516

EIN/Tax ID:
DUNS: N/A
Number of Employees:
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No