USA flag logo/image

An Official Website of the United States Government

IN MAN

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
4808
Program Year/Program:
1986 / SBIR
Agency Tracking Number:
4808
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Epoch Pharmaceuticals, Inc.
1725 220TH ST SE, 104 Bothell, WA 98052
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1986
Title: IN MAN
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

IN THIS PROJECT, SYNTHETIC SHORT DNA PROBES WILL BE DEVELOPED FOR THE DIAGNOSIS OF SPECIFIC TYPES OF HUMAN PAPILLOMAVIRUS (HPV) INFECTIONS. SHORT DNA PROBES OF 20 TO 30 NUCLEOTIDES HAVE THE DESIRABLE PROPERTIES OF HIGH SPECIFICITY, FAST REACTION KINETICS, POTENTIAL HIGH SENSITIVITY, AND THE ABILITY TO BE SYNTHESIZED RAPIDLY IN LARGE AMOUNTS AT LOW COST. THEIR INHERENT HIGH SPECIFICITY IS AN IMPORTANT ATTRIBUTE. IT IS POSSIBLE TO DESIGN IN SITUHYBRIDIZATION ASSAYS THAT CAN DETECT AND DISCRIMINATE ANY OFTHE HPV TYPES PRESENTLY IDENTIFIED. WITH SHORT DNA PROBES, THE RESEARCHERS WILL DEMONSTRATE THAT A UNIVERSAL FORMAT FORIN SITU HYBRIDIZATION CAN BE DEVELOPED USING 2.4 M TETRA- ETHYLAMMONIUM CHLORIDE (TEACL) IN THE HYBRIDIZATION OR WASH STEPS. THIS SALT SOLUTION ALLOWS HYBRIDIZATION AND HYBRID MELTING REACTIONS TO OCCUR SOLELY AS A FUNCTION OF PROBE LENGTH. HENCE, SHORT PROBES OF THE SAME LENGTH (E.G., 25 MERS) WILL HAVE THE SAME HYBRIDIZATION AND MELTING PROPERTIES. IN ADDITION, THE PROJECT WILL DEMONSTRATE THAT AGITATION OF THE HYBRIDIZATION AND WASH STEPS WITH ULTRA- SOUND WILL SIGNIFICANTLY DECREASE THE TIME REQUIRED FOR THE IN SITU DETECTION OF THE VARIOUS TYPES OF HPV FROM THE 24 TO48 HOURS NOW REQUIRED. THE USE OF SHORT PROBES IN CONJUNCTION WITH TEACL SALTS AND ULTRASOUND IS EXPECTED TO BE IMPORTANT IN THE COMMERCIALIZATION OF DNA PROBE TECHNOLOGY FOR USE IN CLINICAL DIAGNOSIS.

Principal Investigator:

Dennis e schwartz
PRINCIPAL INVESTIGATOR
2064858566

Business Contact:

Small Business Information at Submission:

Microprobe Corp
1725 220th Street Se #104 Bothell, WA 98021

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No