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A DNA PROBE FOR THE DIAGNOSIS OF CAMPYLOBACTER PYLORIDIS GASTRITIS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
4889
Program Year/Program:
1988 / SBIR
Agency Tracking Number:
4889
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Epoch Pharmaceuticals, Inc.
1725 220TH ST SE, 104 Bothell, WA 98052
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1988
Title: A DNA PROBE FOR THE DIAGNOSIS OF CAMPYLOBACTER PYLORIDIS GASTRITIS
Agency: HHS
Contract: N/A
Award Amount: $487,000.00
 

Abstract:

THE ROLE OF A SPECIFIC BACTERIUM AS THE POSSIBLE ETIOLOGIC AGENT OF GASTRITIS OR PEPTIC ULCER IS SUPPORTED BY A GROWINGSCIENTIFIC LITERATURE. HOWEVER, THE DIFFICULTY OF CONVENT- IONAL SAMPLING AND DIAGNOSTIC TECHNIQUES HAS RESTRICTED ADVANCES IN DISEASE DIAGNOSIS. THE PURPOSE OF THIS PHASE I PROJECT IS TO DEVELOP A DNA PROBE FROM THE CHROMOSOMAL DNA OF CAMPYLOBACTER PYLORIDIS, A MICROORGANISM RECENTLY SHOWN TO BE ASSOCIATED WITH GASTRITIS AND PEPTIC ULCER. THE 32P- LABELED PROBE WILL BE HYBRIDIZED TO CULTURED BACTERIAL DNA IMMOBILIZED ON NITROCELLULOSE. HYBRIDIZATION SPECIFICITY WILL BE EVALUATED IN A STANDARD NACL SOLUTION AND IN A NEW SALT SOLUTION, TETRAETHYLAMMONIUM CHLORIDE (TEACL). THE TEACL SYSTEM ABOLISHES THE PREFERENTIAL MELTING OF A-T VS. G-C BASE PAIRS IN DNA HYBRIDS, YIELDING A SIGNIFICANT SHARP-ENING OF THE MELTING PROFILE (<1C IN TEACL VS. 5-15C IN NACL), THUS ENHANCING PROBE SPECIFICITY. A DNA PROBE IN A TEACL SALT SYSTEM ALLOWS THE DEVELOPMENT OF A SENSITIVE AND SPECIFIC DNA PROBE ASSAY. THE LONG-TERM OBJECTIVE OF THE PROJECT IS TO PROVIDE A SIMPLE, RAPID, ACCURATE, AND LOW- COST METHOD FOR THE DETECTION OF CAMPYLOBACTER PYLORIDIS IN CLINICAL ISOLATES FROM PATIENTS WITH GASTRITIS OR PEPTIC ULCER. A DIAGNOSTIC TEST WILL BE PRODUCED COMMERCIALLY FOR BY CLINICAL DIAGNOSTIC LABORATORIES.

Principal Investigator:

Dennis E Schwartz
Principal Investigator
2064858566

Business Contact:

Small Business Information at Submission:

Microprobe Corp.
1725 220th Street Southease Suite 104 Bothell, WA 98021

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No