USA flag logo/image

An Official Website of the United States Government

DNA PROBES FOR THE DIAGNOSIS OF BACTERIAL VAGINOSIS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
7093
Program Year/Program:
1989 / SBIR
Agency Tracking Number:
7093
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Epoch Pharmaceuticals, Inc.
1725 220TH ST SE, 104 Bothell, WA 98052
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1989
Title: DNA PROBES FOR THE DIAGNOSIS OF BACTERIAL VAGINOSIS
Agency: HHS
Contract: N/A
Award Amount: $499,906.00
 

Abstract:

THE ROLE OF FIVE BACTERIA AND A MYCOPLASMA AS THE ETIOLOGIC AGENTS OF BACTERIAL VAGINOSIS (BV) IS SUPPORTED BY GROWING SCIENTIFIC DATA. STANDARD METHODS ARE INADEQUATE FOR THE ROUTINE DIAGNOSIS OF THIS DISEASE. EIGHT HUNDRED TO 1,000 NUCLEOTIDES OF THE 16S RIBOSOMAL RNA (RRNA) OF EACH BV MICROORGANISM WILL BE SEQUENCED. SHORT DNA PROBES WILL BE SYNTHESIZED TO DETECT THE 16S RRNA OF EACH BV MICROORGANISM.32P-LABELED DNA PROBES WILL BE HYBRIDIZED AGAINST HIGH-MOLECULAR-WEIGHT RNA AND DNA ISOLATED FROM INDIVIDUAL PURE CULTURES. HYBRIDIZATION SPECIFICITY OF EACH DNA PROBE WILL BE EVALUATED IN TETRAETHYLAMMONIUM CHLORIDE AND TETRAMETHYLAMMONIUM CHLORIDE SOLUTIONS TO ABOLISH THE PREFERENTIAL MELTING OF A-T VERSUS G-C BASE PAIRS IN DNA HYBRIDS, YIELDING HYBRIDIZATIONS THAT ARE ONLY CHAIN-LENGTH DEPENDENT. IN THESE SOLUTIONS, MIXTURES OF PROBES OF THE SAME LENGTH, BUT DIFFERING IN SEQUENCE, WILL EXHIBIT IDENTICAL HYBRIDIZATION PROPERTIES. THESE SALT SOLUTIONS WILL ALSO ALLOW SIMULTANEOUS USE OF MULTIPLE PROBES (OF THE SAME LENGTH) AND WILL ALLOW THE DEVELOPMENT OF A UNIVERSAL HYBRIDIZATION FORMAT. THE LONG-TERM OBJECTIVE IS TO PROVIDE A RAPID AND ACCURATE DIAGNOSTIC TEST FOR BV.

Principal Investigator:

Diana K Sheiness Phd
2064858566

Business Contact:

Small Business Information at Submission:

Microprobe Corp.
1725 220th St Southeast #104 Bothell, WA 98021

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No