USA flag logo/image

An Official Website of the United States Government

CROSS-LINKING OLIGONUCLEOTIDES FOR CHEMOTHERAPY

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
7153
Program Year/Program:
1989 / SBIR
Agency Tracking Number:
7153
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Epoch Pharmaceuticals, Inc.
1725 220TH ST SE, 104 Bothell, WA 98052
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1989
Title: CROSS-LINKING OLIGONUCLEOTIDES FOR CHEMOTHERAPY
Agency: HHS
Contract: N/A
Award Amount: $412,000.00
 

Abstract:

SHORT OLIGODEOXYNUCLEOTIDES INCORPORATING NUCLEOTIDES MODIFIED BY THE ADDITION OF REACTIVE CROSS-LINKING ARMS WILLBE SYNTHESIZED. THESE OLIGONUCLEOTIDES SHOULD HYBRIDIZE WITH TARGET SEQUENCES IN MRNA OR DNA AND, ONCE BOUND, SHOULDCOVALENTLY CROSS-LINK WITH THE COMPLEMENTARY SEQUENCE VIA THE INCORPORATED MODIFIED NUCLEOTIDE. IN PARTICULAR, DERIVATIVES OF 4-AMINOPYRAZOLO(3,4-D)PYRIMIDINE, WITH A HALOALKYL OR SIMILAR SUBSTITUENT IN THE 3-POSITION, WILL BE PREPARED. THESE DERIVATIVES ARE ISOSTERIC WITH A 7-SUBSTITUTED PURINE AND SHOULD BE CAPABLE OF CROSS-LINKING WITH THE 7-POSITION OF A PURINE IN AN ADJACENT BASE PAIR OF THE COMPLEMENTARY STRAND. THIS MODIFIED NUCLEOSIDE WILL BE CONVERTED INTO A BLOCKED PHOSPHORAMIDITE AND INCORPORATED INTO AN OLIGONUCLEOTIDE ON A DNA SYNTHESIZER. THIS OLIGONUCLEOTIDE WILL BE TESTED FOR COVALENT CROSS-LINKING TO COMPLEMENTARY AND NONCOMPLEMENTARYDNA RESTRICTION FRAGMENTS. SUCH ANTISENSE, CROSS-LINKABLE OLIGONUCLEOTIDES, DESIGNED TO MINIMIZE HOST TOXICITY, MAY BE USEFUL AS CHEMOTHERAPEUTIC AGENTS FOR INFECTIOUS DISEASESAND CANCER. THEY WILL BE TARGETED AT THE MRNA OR DNA OF MICROORGANISMS OR OF ANY CANCER TISSUE IN WHICH ARREARRANGEDONCOGENE IS EXPRESSED.

Principal Investigator:

Rich B Meyer Jr Phd
2064858566

Business Contact:

Small Business Information at Submission:

Microprobe Corp.
1725 220th St Southeast #104 Bothell, WA 98021

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No