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DNA PROBE TEST TO DETECT ORAL CANDIDA IN AIDS PATIENTS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
13636
Program Year/Program:
1990 / SBIR
Agency Tracking Number:
13636
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Epoch Pharmaceuticals, Inc.
1725 220TH ST SE, 104 Bothell, WA 98052
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1990
Title: DNA PROBE TEST TO DETECT ORAL CANDIDA IN AIDS PATIENTS
Agency: HHS
Contract: N/A
Award Amount: $49,988.00
 

Abstract:

THIS RESEARCH WILL PROVIDE THE FOUNDATION FOR A RAPID, INEXPENSIVE, NON-CULTURE-BASED TEST FOR THE QUANTITATIVE DETECTION OF ORAL CANDIDA ALBICANS IN HIV-POSITIVE PATIENTS. THE PRIMARY EXPERIMENTAL OBJECTIVE IS TO CREATE A SET OF SHORT SYNTHETIC OLIGODEOXYNUCLEOTIDE PROBES THAT WILL HYBRIDIZE SPECIFICALLY WITH THE 16S RRNA OF C. ALBICANS. SPECIFICITY WILL BE VERIFIED BY HYBRIDIZATION OF EACH (32)P-LABELED PROBE TO SLOT BLOT FILTERS CONTAINING NUCLEIC ACID SAMPLES FROM OVER 50 MICROORGANISMS THAT ARE FOUND FREQUENTLY IN THE OROPHARYNX. FURTHERMORE, THE (32)-LABELEDPROBES WILL BE USED TO MEASURE LEVELS OF C. ALBICANS IN SAMPLES FROM IMMUNOCOMPROMISED PATIENTS. PROBE RESULTS WILL BE COMPARED WITH CONVENTIONAL DIAGNOSTIC EVALUATIONS OF CANDIDIASIS IN THESE SAME PATIENTS. DURING PHASE II OF THIS PROJECT, THESE PROBES WILL BE USED TO DEVELOP A RAPID COLORIMETRIC ASSAY FOR DETECTING AND MONITORING C. ALBICANS IN PERSONS AT HIGH RISK FOR DEVELOPING ACQUIRED IMMUNODEFICIENCY SYNDROME (AIDS). THE FINAL PRODUCT WILL BE A QUANTITATIVE TEST FOR THE CLINICAL LABORATORY THAT CAN BE PERFORMED WITHIN 3 HOURS OR LESS AT ROOM TEMPERATURE AND THAT CAN BE USED AS AN ALTERNATIVE FOR THE MUCH LENGTHIER PROCEDURE OF CULTURING FOR C. ALBICANS.

Principal Investigator:

Diana K Sheiness
2064858566

Business Contact:

Small Business Information at Submission:

Microprobe Corp
1725 220th St Se #104 Bothell, WA 98021

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No