USA flag logo/image

An Official Website of the United States Government

NUCLEAR TARGETING OF OLIGONUCLEOTIDE-PEPTIDE CONJUGATES

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
13637
Program Year/Program:
1992 / SBIR
Agency Tracking Number:
13637
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Epoch Pharmaceuticals, Inc.
1725 220TH ST SE, 104 Bothell, WA 98052
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1992
Title: NUCLEAR TARGETING OF OLIGONUCLEOTIDE-PEPTIDE CONJUGATES
Agency: HHS
Contract: N/A
Award Amount: $499,629.00
 

Abstract:

THIS PHASE I STUDY WILL EXPLORE THE THERAPEUTIC POSSIBILITIES OF ANTISENSE OLIGODEOXYNUCLEOTIDES THAT HAVE BEEN COVALENTLY CONJUGATED TO SIGNAL PEPTIDES THAT CAN DIRECT THE TRANSPORT OF PROTEINS INTO THE NUCLEUS. IN RECENT YEARS, 16 PEPTIDE SEQUENCES WITH NUCLEAR TARGETING PROPERTIES HAVE BEEN IDENTIFIED. THREE OF THESE PEPTIDES WILL BE SYNTHESIZED AND CONJUGATED TO ANTISENSE OLIGODEOXYNUCLEOTIDES THAT INTERFERE WITH SURFACE ANTIGEN PRODUCTION IN HEPATITIS B VIRUS. EACH PEPTIDE-OLIGONUCLEOTIDE CONJUGATE WILL BE LABELED WITH RADIOACTIVE PHOSPHORUS AND TESTED FOR ITS ABILITY TO LOCALIZE TO THE NUCLEUS IN A CELL-FREE PREPARATION OF RAT LIVER NUCLEI. UNMODIFIED OLIGONUCLEOTIDES OF VARIOUS LENGTHS WILL ALSO BE RADIOLABELED, AND THEIR RATE OF NUCLEARUPTAKE/DIFFUSION WILL BE EVALUATED. THE PEPTIDE-OLIGONUCLEOTIDE CONJUGATES WILL ALSO BE EXAMINED FOR IMPROVED ANTISENSE EFFECT IN HEPATITIS B-INFECTED (HEP3B) CELLS. THESE EXPERIMENTS WILL CONSTITUTE A FEASIBILITY STUDY FOR THE DEVELOPMENT OF PEPTIDE-OLIGONUCLEOTIDE CONJUGATES THAT COULD BE IMPORTANT FOR THE THERAPEUTIC TREATMENT OF DISEASESCAUSED BY INFECTION WITH HUMAN IMMUNODEFICIENCY VIRUS, HEPATITIS B, HERPES VIRUSES, OR OTHER VIRUSES.

Principal Investigator:

Michael W Reed
2064858566

Business Contact:

Small Business Information at Submission:

Microprobe Corp.
1725 220th St Se #104 Bothell, WA 98021

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No