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HIV REPORTER CELL LINE FOR SCREENING ANTI-SENSE AGENTS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
16307
Program Year/Program:
1991 / SBIR
Agency Tracking Number:
16307
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Epoch Pharmaceuticals, Inc.
1725 220TH ST SE, 104 Bothell, WA 98052
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1991
Title: HIV REPORTER CELL LINE FOR SCREENING ANTI-SENSE AGENTS
Agency: HHS
Contract: N/A
Award Amount: $50,000.00
 

Abstract:

A SECRETABLE ALKALINE PHOSPHATASE (SPAP) REPORTER GENE WILL BE SUBSTITUTED FOR A PORTION OF THE ENV GENE IN AN HIV PROVIRAL DNA CLONE. EXPRESSION OF THE REPORTER GENE WILL BE REGULATED BY A COMPLETE COMPLEMENT OF VIRAL CIS AND TRANS ACTING CONTROL ELEMENTS, SUCH THAT THE LEVEL OF EXTRACELLULAR ALKALINE PHOSPHATASE WILL SERVE AS AN INDICATOR OF VIRAL GAG, POL, AND ENV REGIONS SUBJECT TO NORMAL REGULATION BY THE VIRAL CODED TAT, REV, AND NEF GENE PRODUCTS. THE H9 CELL LINE WILL BE TRANSFECTED WITH THIS CONSTRUCT AND THE TRANSIENT EXPRESSION OF SPAP, P24, AND REVERSE TRANSCRIPTASE CHARACTERIZED. THE FEASIBILITY OF USING THE SPAP AS A MARKER TO DETECT ANTIVIRAL ACTIVITY WILL BE ASSESSED BY MONITORING THE EFFECT OF THREE ANTISENSE OLIGONUCLEOTIDES (ODNS) TARGETED TO CRITICAL SITES ON THE HIV GENOME IN THE TRANSFECTED H9 CELLS.

Principal Investigator:

Gamper, Howard B
Principal Investigator
2064858566

Business Contact:

1 r43 ai31315-01
Small Business Information at Submission:

Microprobe Corpon
1725 220th Street Se, #104 Bothell, WA 98021

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No