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ANTI-SENSE INHIBITION OF HBSAG EXPRESSION IN HEP G2 CELL

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
16298
Program Year/Program:
1991 / SBIR
Agency Tracking Number:
16298
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Epoch Pharmaceuticals, Inc.
1725 220TH ST SE, 104 Bothell, WA 98052
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1991
Title: ANTI-SENSE INHIBITION OF HBSAG EXPRESSION IN HEP G2 CELL
Agency: HHS
Contract: N/A
Award Amount: $49,936.00
 

Abstract:

UNIQUELY MODIFIED ANTI-SENSE OLIGODEOXYNUCLEOTIDES (ODNS) TARGETED TO THE HEPATITIS B SURFACE ANTIGEN TRANSCRIPT WILL BE EVALUATED IN A DIFFERENTIATED HEPATOMA CELL LINE WHICH SUPPORTS THE CONSTITUTIVE PRODUCTION OF INFECTIOUS DANE PARTICLES. INHIBITION OF SURFACE ANTIGEN PRODUCTION WILL BE MONITORED BY ANALYZING ALIQUOTS OF THE MEDIA FOR HEPATITIS B SURFACE ANTIGEN USING A COMMERCIALLY AVAILABLE ELISA KIT. ODN MODIFICATIONS TO BE EXAMINED WILL CONSIST OF ALKYL SIDE ARMS TERMINATING IN AMINO, CHOLESTEROL, OR ACRIDINE GROUPS. THESE ARMS WILL BE INTRODUCED SINGLY OR IN COMBINATION AT 5', 3', AND INTERNAL SITES OF THE ODN. SELECTED ODNS WILL BE COMPLEXED WITH AN ASIALOOROSOMUCOID-POLY-L-LYSINE CONJUGATE PREVIOUSLY SHOWN TO PROMOTE RECEPTOR-MEDIATED INTERNALIZATION OF DNA IN HEPATOCYTES. THE EFFECT OF THIS DELIVERY SYSTEM ON THE POTENCY OF THE ANTI-SENSE EFFECT AND ON THE STABILITY AND CELLULAR UPTAKE OF EACH ODN WILL BE DETERMINED.

Principal Investigator:

Gamper, Howard B
Principal Investigator
2064858566

Business Contact:

1 r43 ai31007-01a1
Small Business Information at Submission:

Microprobe Corpora- Tion
1725 220th Street Se, #104 Bothell, WA 98021

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No