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DNA PROBES TO DETECT PREDISPOSITION TO TYPE I DIABETES

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
19209
Program Year/Program:
1994 / SBIR
Agency Tracking Number:
19209
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Epoch Pharmaceuticals, Inc.
1725 220TH ST SE, 104 Bothell, WA 98052
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1994
Title: DNA PROBES TO DETECT PREDISPOSITION TO TYPE I DIABETES
Agency: HHS
Contract: N/A
Award Amount: $470,000.00
 

Abstract:

INSULIN-DEPENDENT DIABETES MELLITUS (TYPE I) OCCURS IN ABOUT 0.5% OF CAUCASIANS. THE INCIDENCE INCREASES OVER 20-FOLD IN INDIVIDUALS WITH THE SPEIFIC SUSCEPTIBILITY GENE HLA-DQ3.2. APPROXIMATELY 70% OF INDIVIDUALS WHO BECOME INSULIN-DEPENDENT DIABETICS CARRY THIS GENE, MAKING IT THE MOST SPECIFIC MARKER KNOWN FOR IDENTIFYING INDIVIDUALS AT RISK. CLEARLY THIS ILLUSTRATES THE NEED FOR SCREENING AND IDENTIFICATION OF THE DQ3.2 GENE. IN THIS WORK, DNA OLIGONUCLEOTIDE PROBES WILL BE USED IN A NYLON BEAD-BASED SANDWICH HYBRIDIZATION ASSAY FOR THE DETECTION OF THE DQ3.2 GENE IN HUMAN LYMPHOCYTES. HYBRIDIZATION WITH TARGET DQ3.2 GENE WILL BE DETECTED USING A CHEMILUMINESCENT SIGNAL SYSTEM. THE PROCEDURES WILL BE DEVELOPED FOR THE RAPID EXTRACTION AND DETECTION OF THE DQ3.2 GENE WITH HIGH SPECIFICITY AND SENSITIVITY.

Principal Investigator:

Nicolaas M J Vermeulen
8004474362

Business Contact:

Small Business Information at Submission:

Microprobe Corp.
1725 220th Street Se 104 Bothell, WA 98021

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No