Biochemistry of Oligomer/Pyrroloindole Conjugates
1 R43 GM52774-01A1,
We have developed the chemistry to conjugate a pyrroloindole tripeptide (CDPI3) analog of CC-1065 t3' ends of any oligodeoxyribonucleotide (ODN). The resultant conjugates hybridize sequence specificaDNA and RNA strands, yet do not interact with double-stranded DNA. The conjugated CDPI3 moiety can sstabilize short duplexes by binding to the minor groove. In light of these results, we will evaluateconjugates as anti sense and antigene agents. The ability of ODN-CDPI3 conjugates to sequence specifreplication, transcription and translation will be investigated using simple in vitro model systems.studies will be initiated to evaluate the potential of these modified ODNs for use as anti sense, anagents.
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Principal Investigator:Igor Kutyavin
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