ORIENTED PROTEIN MICROARRAY FOR FUNCTIONAL STUDIES

DESCRIPTION (provided by applicant):A novel method is proposed for the
fabrication of protein microarrays using metal coordination chemistry and
poly-histidine tagged protein molecules. The proposed method is aimed at
microarrays with highly selective and facile linking chemistry, with uniform
and controllable orientation of immobilized protein molecules, and with
proteins in native states and easily accessible by targets in the solution.
Preliminary experiments using 6x-His terminated green-fluorescence protein
(GFP) demonstrated the feasibility and potential of the proposed approach. We
will establish the advantage of an oriented protein microarray in
protein-ligand interaction studies over randomly oriented arrays fabricated by
existing methods. We also propose to explore protein immobilization without
purification on the highly selective metal ion surface.
PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE