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Oriented Protein Microarray for Functional Studies

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
60104
Program Year/Program:
2004 / SBIR
Agency Tracking Number:
RR017130
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
MICROSURFACES INC
3913 Todd Lane SUITE 102 Austin, TX 78744-
View profile »
Woman-Owned: Yes
Minority-Owned: Yes
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 2004
Title: Oriented Protein Microarray for Functional Studies
Agency: HHS
Contract: 2R44RR017130-02
Award Amount: $733,438.00
 

Abstract:

DESCRIPTION (provided by applicant): The proposed research is aimed at developing protein microarrays as a superior technology oriented and viable product for the proteomics market. During Phase-I research, the PI and coworkers have successfully demonstrated the feasibility of fabricating protein microarrays with high selectivity and specificity, with uniform and controllable orientation of immobilized protein molecules, and with proteins in native states and easily accessible by targets in the solution. More importantly, preliminary research established not only the advantage of this chemistry in terms of selective protein immobilization without the need of pre-purification but also the necessity of orientational control in ensuring activity of the native state. The latter was demonstrated by the observation that enzymatic activity of oriented sulfotransferases on the surface was the same as that of solution phase enzymes, while randomly oriented enzymes on the surface showed less than 20% of the activity. Under Phase II funding, we propose (1) to establish the generality of oriented proteins on the surface in maintaining native activity; (2) to develop methods for on-chip refolding of immobilized proteins; (3) to further develop surface coating technology for commercialization. The success of Phase-II research will lead to the launch of a superior surface coating technology for protein microarrays with higher performance at potentially lower cost than current technology in the protein microarray market, which is very important for future large scale drug screening and disease diagnosis.

Principal Investigator:

Athena Guo
7637880255
ATHENA@MEMSURFACE.COM

Business Contact:

Athena Guo
7637880255
ATHENA@MEMSURFACE.COM
Small Business Information at Submission:

MICROSURFACES, INC.
MICROSURFACES, INC. 4220 CENTRAL AVE NE, STE 101 MINNEAPOLIS, MN 55421

EIN/Tax ID: 411991341
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No