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CONTRIBUTION OF SURFACE PROPERTIES TO ANTIBODY IMMOBILIZATION

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
4941
Program Year/Program:
1987 / SBIR
Agency Tracking Number:
4941
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
Midwest Research
180 North Bishops Way Brookfield, WI 53005
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1987
Title: CONTRIBUTION OF SURFACE PROPERTIES TO ANTIBODY IMMOBILIZATION
Agency: HHS
Contract: N/A
Award Amount: $239,000.00
 

Abstract:

THE IMMOBILIZATION OF PROTEINS ON INSOLUBLE SURFACES IS IMPORTANT IN DIAGNOSTIC INSTRUMENTATION AND IN VIVO MONITOR-ING. IMPROVEMENTS IN THE SURFACE ANTIBODY INTERACTIONS SHOULD RESULT IN MORE EFFICIENT APPROACHES TO IMMUNO- DIAGNOSTICS. THIS WOULD BE ESPECIALLY USEFUL IN THE FIELD OF TUMOR MARKER DETECTION WHERE PANELS OF ANTIGENS MAY BE NEEDED FOR PROPER DIAGNOSIS. THIS RESEARCH IS THE DEVELOPMENT OF A MULTIPLE IMMUNOLOGICAL TEST WITH AN ELECTRONIC ENDPOINT. THE RESEARCH WILL INVOLVE THE USE OF ANTIGEN OR ANTIBODY-LABELED COLLOIDAL GOLD PARTICLES TO GENERATE AN ELECTRONIC ENDPOINT. A DIAGNOSTIC ELEMENT WILL BE REFINED SO THAT THE ANTIBODY-ANTIGEN REACTION CAUSES THE COMPLETION OF AN OPEN ELECTRICAL CIRCUIT. THIS MODEL NECESSITATES AN ELECTRICALLY INSULATING BASE SURFACE FOR PROTEIN ADHESION. A PAIR OF CONDUCTORS IS PLACED ON THE DEFINING A CHANNEL BETWEEN THEM. THE ANTIGEN IS PLACED IN THE CHANNEL. THE GOLD-TAGGED ANTIBODY WILL BOND TO THE SPECIFIC ANTIGEN, FORMING A CONDUCTIVE BRIDGE ACROSS THE CHANNEL FOR A POSITIVE REACTION. THIS NEW TYPE OF DEVICE COULD REPLACE THE CURRENTLY USED ENZYME IMMUNOASSAY, IMMUNO-FLUORESCENCE, AND RADIOIMMUNOASSAY TECHNIQUES, WHICH ARE RELIABLE BUT SLOW, TEDIOUS, AND RESTRICTED TO SINGLE TESTS PER SAMPLE. THE COMMERCIAL APPLICATIONS OF A MULTIPLE TEST ENCOMPASS HUMAN DIAGNOSTICS SUCH AS CANCER AND ALLERGY AS WELL AS VETERINARY AND FORENSIC DIAGNOSTICS.

Principal Investigator:

Susan J Mroczkowski
Principal Investigator
4145272260

Business Contact:

Small Business Information at Submission:

Midwest Research Microscopy
5510 West Florist Avenue Milwaukee, WI 53218

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No