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LARGE-SCALE PRODUCTION OF AVIAN MYELOBLASTOSIS VIRUS

Award Information

Agency:
Department of Health and Human Services 		Branch:
N/A 		Award ID:
7157 		Program Year/Program:
1987 / SBIR
Agency Tracking Number:
7157 		Solicitation Year:
N/A 		Solicitation Topic Code:
N/A 		Solicitation Number:
N/A
Small Business Information
Molecular Biology Resources
5520 West Burleigh Street Milwaukee, WI 53210
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1987
Title: LARGE-SCALE PRODUCTION OF AVIAN MYELOBLASTOSIS VIRUS
Agency: HHS
Contract: N/A
Award Amount: $41,000.00
 

Abstract:

AVIAN MYELOBLASTOSIS VIRUS (AMV), A SOURCE OF REVERSE TRANSCRIPTASE FOR USE IN RECOMBINANT DNA WORK, IS CURRENTLY PRODUCED COMMERCIALLY FROM THE PLASMA OF LEUKEMIC CHICKS. PRODUCTION INVOLVES INOCULATION OF SEVERAL HUNDRED DAY-OLD CHICKS WITH AMV AND HARVEST OF VIRUS-CONTAINING BLOOD BY HEART PUNCTURE 7 TO 10 DAYS LATER. THE RESEARCH PROPOSED WILL INVESTIGATE THE FEASIBILITY OF COMMERCIAL-SCALE PRODUCTION OF AMV FROM CELL CULTURES OF CHICKEN MYELOBLASTS.PROCEDURES FOR IN VITRO TRANSFORMATION OF CHICKEN MACROPHAGELINEAGE CELLS WITH AMV AND THE IN VITRO GROWTH OF THE TRANSFORMED CELLS ON A SMALL SCALE ARE WELL KNOWN. LARGE-SCALE CULTURE OF AMV-TRANSFORMED MYELOBLASTS HAS ALSO BEEN ACHIEVED, BUT WITH SPECIALLY CONSTRUCTED APPARATUS. THE PRESENT PROPOSAL DISCUSSES ADAPTATION OF RECENT ADVANCESIN CELL CULTURE CONDITIONS AND APPARATUS FOR LARGE-SCALE CELL CULTURE TO PRODUCTION OF AMV. SUCCESSFUL DEVELOPMENT OF THE CELL CULTURE METHOD WILL GIVE A PROCEDURE THAT IS BOTH MORE ECONOMICAL AND MORE HUMANE THAN THE PRESENT IN VIVO METHOD.

Principal Investigator:

Richard f piehl phd
4148717199

Business Contact:

Small Business Information at Submission:

Molecular Biology Resources
5520 West Burleigh Street Milwaukee, WI 53210

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No