The long term goal of this research is to develop synthetic dna hybridization probes to detect individuals at risk for cancer. dna hybridization has proven highly effective as a research diagnostic tool; however, conventional dna hybridization probes are expensive to commercialize, since they depend… More

Since june, 1981, over 700 cases of aids have been reported to the centers for disease control. most cases have involved homosexual or bisexual men; the remainder involved heterosexual men and women, haitians, and intravenous drug abusers. the etiology of the disease is probably a variously… More

The long term goal of this research is to develop synthetic dna hybridization probes to detect viral infections. dna hybridization has proven highly effective in detecting viruses, but current methods for making dna hybridization probes are expensive to commercialize since they depend on… More

Rotaviruses are the major cause of serious infantile diarrhea. this pathogen accounts for 4060% of the diagnosed cases of gastroenteritis in children under the age of two years. diagnosis is currently made by elisa or by electron microscopy. the latter is impractical for the small laboratory, and… More

The long-term goal of this research is to develop a highly sensitive nonradioactive detection method for dna hybrids which will be useful with clinical samples. dna hybridization probes are proving to be useful for the detection of specific bacteria and viruses as well as for the detection of… More

Our goal is to develop a rapid, simple dna hybridization system for use in clinical samples. current methods for performing hybridizations (e.g., nitrocellulose dot blots) are difficult to apply to crude samples and require protracted sample preparation. one promising solution is the hybridization… More

This research is directed toward developing means of utilizing magnetic microspheres to rapidly and reliably separate target cells. initially the target cell will be peripheral blood t-lymphocytes and the work will evaluate the ability to deplete this cell population. later, the system will be… More

The goal of this research is to develop a rapid and highly sensitive direct fluorescent detection system for dna hybrids. dna hybridization probes are useful for detecting specific pathogens and genetic alterations. radioautography, currently the most sensitive detection technique, can visualize 15… More

Dna hybridization probes are useful for detecting specific bacteria, viruses and genetic abnormalities. in order to fully utilize the potential of such probes, a rapid, sensitive, nonradioactive detection method is needed. radioautography can visualize 15 picograms of target dna in overnight… More

Until recently, cardiac ultrasound has offered no method of evaluating myocardial perfusion other than assessment of theend results of ischemia, such as wall motion abnormalities. recent attempts at developing an effective contrast agent to measure myocardial perfusion have resulted in the… More

An in situ dna hybridization assay suitable for detecting target nucleic acid in single intact cells by automatic flowanalysis will be investigated. protocols for fixing cells in suspension and dna denaturation will be developed. emphasis will be placed on minimizing cell clumping for compatibility… More