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AFFINITY PURIFICATION OF PARAINFLUENZA VIRUS GLYCOPROTEINS

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
2939
Program Year/Program:
1986 / SBIR
Agency Tracking Number:
2939
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
MOLECULAR ENGINEERING CORP.
620 SIERRA MADRE BLVD SAN MARINO, CA 91108
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1986
Title: AFFINITY PURIFICATION OF PARAINFLUENZA VIRUS GLYCOPROTEINS
Agency: HHS
Contract: N/A
Award Amount: $498,100.00
 

Abstract:

IT IS PROPOSED TO PURIFY THE TWO ENVELOPE GLYCOPROTEINS, HN AND F, FROM HUMAN PARAINFLUENZA TYPE 3 (PI3) VIRUS BY AFFINITY CHROMATOGRAPHY USING SPECIFIC MONOCLONAL ANTIBODIES AND TO INCORPORATE THEM INTO LIPID VESICLES. THE EXPERIMENTAL APPROACH INVOLVES IMMOBILIZATION OF SPECIFIC MONOCLONAL ANTIBODY ON A SEPHAROSE 4B MATRIX. PURIFIED VIRUS OR INFECTED CELLS WILL BE USED AS THE SOURCE OF ANTIGENS FOR ADSORPTION AND PURIFICATION OF SPECIFIC GLYCOPROTEINS THROUGH AFFINITY COLUMNS. THE TWO PURIFIED ANTIGENS WILL BE INCORPORATED IN DEFINED PROPORTIONS INTO LIPID VESICLES. THIS RESEARCH WILL FACILITATE THE DEVELOPMENT OF MORE EFFECTIVE VACCINES AND WILL LEAD TO A PHASE II PROPOSAL IN WHICH THE EFFICACY OF THE RECONSTITUTED GLYCOPROTEIN-CONTAINING VESICLES WILL BE EVALUATED AS A VACCINE IN EXPERIMENTAL ANIMALS.

Principal Investigator:

Ranjit Ray
Principal Investigator
2059345036

Business Contact:

Small Business Information at Submission:

Molecular Engineering Associat
2001 Park Place Birmingham, AL 35203

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No