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Cancer drug discovery using disordered protein targets

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
65358
Program Year/Program:
2003 / SBIR
Agency Tracking Number:
CA097568
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
MOLECULAR KINETICS, INC.
7201 Chablis Court Indianapolis, IN 46278-1540
View profile »
Woman-Owned: Yes
Minority-Owned: Yes
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2003
Title: Cancer drug discovery using disordered protein targets
Agency: HHS
Contract: 1R43CA097568-01A1
Award Amount: $100,000.00
 

Abstract:

DESCRIPTION (provided by applicant): A novel technique for cancer drug discovery, "Disordered Protein Phage Display Assay", is proposed. This new technique will inject new avenues to cancer drug discovery methods since disordered proteins are only recently proven to have promising drug-targetable functions. The basic principle is based on the fact that disordered regions of proteins are more sensitive to protease digestion than ordered regions, whether naturally ordered or induced to be ordered. In Phase I, a model system using paclitaxel and bcl-2 will be used to demonstrate feasibility. This system is chosen because paclitaxel is a known anti-cancer drug and binds to the disordered loop of bcl-2. First, the hypothesis that small molecule (paclitaxel) binding to disordered target (bcl-2) inhibits its degradation by protease will be confirmed. Then the disordered region of bcl-2 will be cloned into a phage display vector and the hybrid will be tested by protease in the presence or absence of paclitaxel. Growth of the phage after protease and paclitaxel exposure indicates protection of the disordered region by paclitaxel. The ultimate final product will be a new methodology and expert analysis to accelerate drug lead identification via interaction with disordered regions of cancer-associated proteins.

Principal Investigator:

A. K. Dunker
3172789650
KEDUNKER@IUPUI.EDU

Business Contact:

Ya-yue Van
3176380244
MAIN@MOLECULARKINETICS.COM
Small Business Information at Submission:

MOLECULAR KINETICS, INC.
MOLECULAR KINETICS, INC. 351 W 10TH ST INDIANAPOLIS, IN 46202

EIN/Tax ID: 911366111
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No