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NOVEL FLUOROGENIC ENZYME SUBSTRATES AND THEIR USES

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
19282
Program Year/Program:
1994 / SBIR
Agency Tracking Number:
19282
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
MOLECULAR PROBES, INC.
MOLECULAR PROBES, INC. 4849 PITCHFORD AVE EUGENE, OR 97402
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 2
Fiscal Year: 1994
Title: NOVEL FLUOROGENIC ENZYME SUBSTRATES AND THEIR USES
Agency: HHS
Contract: N/A
Award Amount: $495,678.00
 

Abstract:

THIS PROJECT INVOLVES DEVELOPMENT OF NOVEL FLUOROGENIC SUBSTRATES AND THEIR BIOANALYTICAL APPLICATIONS. SUPPORT IS SPECIFICALLY REQUESTED FOR SYNTHESIS OF FLUOROGENIC ENZYME SUBSTRATES THAT YIELD INTENSELY FLUORESCENT PRECIPITATES NEAR THE SITE OF ENZYMATIC ACTIVITY. WORK IN PROGRESS HAS DEMONSTRATED A SIGNIFICANT POTENTIAL FOR THESE REAGENTS FOR ULTRASENSITIVE DETECTION IN HISTOCHEMISTRY, MOLECULAR BIOLOGICAL TECHNIQUES INCLUDING DNA HYBRIDIZATION, DETECTION OF LOW ABUNDANCE CELLULAR RECEPTORS AND DETECTION OF ENZYMES IN LIVING CELLS. ENZYMATIC AMPLIFICATION OF THE SIGNAL RESULTS IN FLUORESCENCE THAT IS ORDERS OF MAGNITUDE GREATER THAN THAT POSSIBLE WITH FLUORESCENT DYE CONJUGATES AND SIGNIFICANTLY GREATER THAN THAT POSSIBLE WITH SUBSTRATES THAT YIELD COLORED PRECIPITATES. SINCE THE FLUORESCENT PRECIPITATES REPRESENT "PURE" DYE WITH NO CARRIER PROTEIN, THE SUBSTRATES CAN POTENTIALLY BE USED TO DETECT ENZYMATIC ACTIVITY WITH HIGH SPATIAL RESOLUTION WHETHER THE ENZYME IS INTRINSICALLY PRESENT IN CELLS OR IS USED AS A TOOL IN A CONJUGATED FORM SUCH AS IN ELISA TECHNIQUES.

Principal Investigator:

Richard P Haugland
5033443007

Business Contact:

Small Business Information at Submission:

Molecular Probes Inc.
4849 Pitchford Avenue Eugene, OR 97402

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No