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A Highly Miniaturized Device For Fully Differentiated, Primary Cell Models of…

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
96347
Program Year/Program:
2010 / SBIR
Agency Tracking Number:
HL103079
Solicitation Year:
N/A
Solicitation Topic Code:
NHLBI
Solicitation Number:
N/A
Small Business Information
BELLBROOK LABS, LLC
5500 NOBEL DR, STE 230 MADISON, WI -
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2010
Title: A Highly Miniaturized Device For Fully Differentiated, Primary Cell Models of Air
Agency: HHS
Contract: 1R43HL103079-01
Award Amount: $391,927.00
 

Abstract:

DESCRIPTION (provided by applicant): There is a strong need for more predictive cellular assays that better represent the tissue that is under investigation. For airway epithelia such a model exists, and uses primary cells grown at an air liquid interface (ALI). A key technical hurdle has been the large cell numbers that are needed to run these assays using cell culture inserts. This method is also very limited with respect to microscopic interrogation. In this proposal we intend to develop a microchamber t hat will enable highly miniaturized airway epithelial assays. This will dramatically increase the number of data points that can be gleaned from a limited set of primary cells. Furthermore, these devices will allow high quality high content imaging to be p erformed, which will provide new levels of information including cilia beat frequency. To this end, the entire device will be made of optical quality thermoplastics. The device consists of very small membrane culturing area with a microchannel below. The d evice is operated simply by placing and removing droplets, so can be used with a handheld pipettor as well as automated liquid handling robotics. We anticipate this device will significantly enhance research regarding in vitro models of airways disease. In addition, other models such as skin and blood brain barrier should be readily adapted to this platform.

Principal Investigator:

Steven A. Hayes
6082274508
STEVE.HAYES@BELLBROOKLABS.COM

Business Contact:

Robert G. Lowery
bob.lowery@bellbrooklabs.com
Small Business Information at Submission:

BELLBROOK LABS, LLC
BELLBROOK LABS, LLC 5500 NOBEL DR, STE 250 MADISON, WI 53711

EIN/Tax ID: 104366135
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No