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Low cost, high density custom peptide microarray synthesis205

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
96217
Program Year/Program:
2010 / SBIR
Agency Tracking Number:
GM093505
Solicitation Year:
N/A
Solicitation Topic Code:
NIGMS
Solicitation Number:
N/A
Small Business Information
BIODISCOVERY, LLC
5692 Plymouth rd ANN ARBOR, MI -
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2010
Title: Low cost, high density custom peptide microarray synthesis205
Agency: HHS
Contract: 1R43GM093505-01
Award Amount: $219,260.00
 

Abstract:

DESCRIPTION (provided by applicant): Peptides play a significant role in the defense mechanisms of the body and binding of cells, bacteria and viruses to surfaces. Combinatorial peptide chemistry has emerged as a powerful tool for mapping receptor-ligand i nteractions in drug discovery applications as well as epitope mapping. There is a huge, but largely unrealized, potential for peptide microarray applications in drug discovery, study of cellular pathways and treatment of tumors. There are two reasons why t he peptide micro arrays have not yet reached their potential: i) the enormous diversity possible with peptide microarrays as well as ii) the high cost of peptide microarrays in comparison to DNA microarrays. In this proposal our goals are to: 1) Develop a highly flexible and fast in situ custom peptide synthesis technology which can lower the cost of peptide microarrays by at least an order of magnitude and reduce the synthesis time to less than 24 hours for peptides containing up to 15 amino acids; 2) Incr ease the density of peptides on a microarray by an order of magnitude to gt10,000/array; 3) Use fluorescent probes as well as high resolution mass spectroscopy to determine sequence purity and stepwise yields for addition of each of the 20 naturally occurr ing amino acids. We have developed a revolutionary light gated oligonucleotide microarray synthesis technology which uses off the shelf reagents and a modified projector to carry out custom microarray synthesis on open or curved surfaces with probe densiti es of up to 500K/glass slide for about one tenth the cost of most commercial microarrays of similar density. In this project we will modify the chemistry and instrumentation used for oligonucelotide microarray synthesis to develop a system for combinatoria l peptide synthesis on open/closed slide surfaces or membranes.

Principal Investigator:

Jeanmarie Rouillard
7347634722
JMROUILL@UMICH.EDU

Business Contact:

Marie J. Rouillard
jmr@biodiscovery-llc.com
Small Business Information at Submission:

BIODISCOVERY, LLC
BIODISCOVERY, LLC 5692 Plymouth rd ANN ARBOR, MI 48105

EIN/Tax ID: 120253660
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No