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High Efficiency Transfection via Nanospearing
- Small Business Information
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Woman-Owned: NoMinority-Owned: NoHUBZone-Owned: No
- Phase 1
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Fiscal Year: 2007Title: High Efficiency Transfection via NanospearingAgency: HHSContract: 1R43EB006249-01A1Award Amount: $398,332.00
Abstract:
DESCRIPTION (provided by applicant): NanoLab is proposing to develop a new biomolecule delivery technique named nanospearing , based on its previously published work. DNA plasmids are immobilized on magnetically susceptible, needle-like nanostructures. Th ese needles can be magnetically accelerated to penetrate ('spear') the cell membrane. The plasmid DNA bound to the nanotubes can be released and expressed in the cells. Two pieces of equipment, called GeneBlender and GeneHammer, are proposed for spearing c ells in suspension and on substrates respectively. Theoretical analysis is to to be used for optimizing the system configuration so that nanotubes can be driven to their highest speed for spearing. Different molecular immobilization strategies will be test ed to achieve the efficient plasmid loading. Upon ressolving the potential pitfalls, the process will be universalized and packaged into a transfection kit. The development will be focused on plasmid DNA delivery at this phase. It is enasaged for applicati ons in vivo, such as drug delivery and vaccination.Small Business Information at Submission:NANOLAB, INC.
NANOLAB, INC. 55 CHAPEL ST NEWTON, MA 02458EIN/Tax ID: 043501871DUNS: N/ANumber of Employees: N/AWoman-Owned: NoMinority-Owned: NoHUBZone-Owned: No
Award Information
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Agency: Department of Health and Human Services |
Branch: N/A |
Award ID: 85596 |
Program Year/Program: 2007 / SBIR |
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Agency Tracking Number: EB006249 |
Solicitation Year: N/A |
Solicitation Topic Code: N/A |
Solicitation Number: N/A |