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DEVELOPMENT OF AN ULTRASENSITIVE DETECTION SYSTEM FOR DNA SEQUENCING

Award Information

Agency:
Department of Energy
Branch:
N/A
Award ID:
14536
Program Year/Program:
1991 / SBIR
Agency Tracking Number:
14536
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
NANOPROBES, INC.
95 HORSEBLOCK RD YAPHANK, NY 11980-
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1991
Title: DEVELOPMENT OF AN ULTRASENSITIVE DETECTION SYSTEM FOR DNA SEQUENCING
Agency: DOE
Contract: N/A
Award Amount: $49,867.00
 

Abstract:

A NEW GOLD PARTICLE HAS BEEN DEVELOPED THAT IS WATER SOLUBLE, CAN BE REACTED WITH NUCLEIC ACIDS IN A SPECIFIC WAY, AND IS HIGHLY SENSITIVE TO SILVER DEVELOPER. THIS SYSTEM HAS EXPERIMENTALLY DEMONSTRATED POTENTIAL TO DETECT 10(-20) MOL OF TARGET. IN PHASE I, BOTH THE COUPLING OF THEGOLD PARTICLE TO NUCLEIC ACIDS AND THE SILVER DEVELOPMENT ISBEING OPTIMIZED. THE LIMIT OF SENSITIVITY AFTER AMPLIFICATION WILL THEN BE DETERMINED. THE GOLD PARTICLE ISBEING APPLIED TO MODEL DNA ASSAY SYSTEMS THAT REQUIRE HIGH SENSITIVITY AND HIGH SPECIFICITY; THE PARTICLE IS THEN AMPLIFIED WITH SILVER DEVELOPER TO EVALUATE ITS PERFORMANCE AS A NUCLEIC ACID PROBE. THE CAPABILITY OF THE GOLD PARTICLE SYSTEM IS BEING EVALUATED IN HYBRIDIZATION ASSAYS AND IN GELS TO DETERMINE WHICH METHOD IS BETTER FOR FRAGMENT DETECTION. ITS PERFORMANCE IN MODELS FOR MULTIPLEXSEQUENCING IS ALSO BEING EXAMINED. THESE TESTS PROVIDE A BASIS FOR EVALUATION OF ITS USE IN STRATEGIES FOR MORE RAPID SEQUENCING RATES THAT INCLUDE SEQUENCING BY HYBRIDIZATION AND MULTIPLEX SEQUENCING.

Principal Investigator:

Dr Frederic R Furuya
Principal Investigator
0

Business Contact:


5162823372
Small Business Information at Submission:

Nanoprobes Inc
Life Sciences Building Rm 008 State University Of New York Stony Brook, NY 11794

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No