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AMPLIFICATION OF SILVER-GOLD IMMUNO- AND DNA PROBES

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
16433
Program Year/Program:
1991 / SBIR
Agency Tracking Number:
16433
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
NANOPROBES, INC.
95 HORSEBLOCK RD YAPHANK, NY 11980-
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1991
Title: AMPLIFICATION OF SILVER-GOLD IMMUNO- AND DNA PROBES
Agency: HHS
Contract: N/A
Award Amount: $49,838.00
 

Abstract:

NEW METHODOLOGIES WILL BE USED FOR THE DETECTION AND QUANTITIZATION OF VERY SMALL AMOUNTS OF PROTEINS AND DNA SEQUENCES. A NEW GOLD PARTICLE WILL BE USED TO LABEL TARGET BIOMOLECULES, THEN STAINED WITH METALLIC SILVER BY EXPOSURE TO AQUEOUS SILVER (I) IONS AND A REDUCING AGENT. THE SILVER WILL THEN BE EXTRACTED AND DETERMINED IN AQUEOUS SOLUTION, USING DYE COUPLING REACTIONS AT A POLYMER SURFACE IN CONJUNCTION WITH SPECTROPHOTOMETRIC MEASUREMENTS. COLORIMETRIC ANALYSIS, CATALYTIC SPECTROPHOTOMETRIC MEASUREMENTS AND ANODIC STRIPPING VOLTAMMETRY WILL ALSO BE USED TO DEVELOP FAST, SIMPLE AMPLIFICATION PROCEDURES FOR THE SILVER-STAINED GOLD LABEL. CALCULATIONS INDICATE THAT SENSITIVITIES OVER 10,000 TIMES GREATER THAN THOSE CURRENTLY AVAILABLE CAN BE ACHIEVED: THIS WOULD ALLOW THE DETECTION OF 1 X 10 TO THE -23 MOLE OF ANTIBODY OR DNA (EQUIVALENT TO 8 X 10 TO THE -19 G OF ALKALINE PHOSPHATASE, OR 1.2 X 10 TO THE -20 G OF A FOUR-BASE OLIGONUCLEOTIDE).

Principal Investigator:

Powell, Richard D
Principal Investigator
5166329235

Business Contact:

1 r43 ca55432-01
Small Business Information at Submission:

Nanoprobes, Inc
Life Sciences Building, Room 0 08 Suny @stony Brook Stony Brook, NY 11794

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No