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MOLECULAR CYTOSKELETAL MICROSCOPY PROBES AND BIOLOGICAL WIRES
- Small Business Information
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Woman-Owned: NoMinority-Owned: NoHUBZone-Owned: No
- Phase 1
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Fiscal Year: 1994Title: MOLECULAR CYTOSKELETAL MICROSCOPY PROBES AND BIOLOGICAL WIRESAgency: NSFContract: N/AAward Amount: $64,993.00
Abstract:
NOVEL PROBES ARE INTRODUCED FOR THE STUDY OF CYTOSKELETAL STRUCTURES AND PROCESSES BY ELECTRON MICROSCOPY. THESE COMPRISE LARGE CLUSTER COMPLEXES OF GOLD, PLANTINUM, AND PALLADIUM 0.8 NM-2.4 NM IN DIAMETER COVALENTLY LINKED TO PHALLOIDIN, CYTOCHALASIN B, COLCHICINE, AND TAXOL VIA A SINGLE REACTIVE FUNCTIONAL GROUP INCORPORATED INTO THEIR LIGAND SPHERES. THE CONJUGATES CAN PENETRATE 20-30 MUM OR MORE INTO CELL SECTIONS AND ACHIEVE SIGNIFICANTLY HIGHER RESOLUTION THAN COLLOIDAL GOLD-ANTIBODY CONJUGATES. THEY ARE MICROINJECTABLE; COMPATIBLE WITH LIVING CELLS; AND USED TO EXTEND THE USE OF ELECTRON MICROSCOPY TO THE STUDY OF ORGANISMS SUCH AS PROCARYOTIC BACTERIA, IN WHICH ANTIBODY PROBES ARE NOT FEASIBLE. COMBINED LABELS INCLUDING BOTH A GOLD CLUSTER AND A FLUORESCENT MOIETY ARE BEING USED TO PREPARE ADDITIONAL PROBES WITH THE SAME MOLECULES, ENABLING CORRELATION OF FLUORESCENCE CONFOCAL MICROSCOPY AND ELECTRON MICROSCOPY DATA FROM A SINGLE LABELING EXPERIMENT. THE PREPARATION OF MOLECULAR WIRES IS BEING ATTEMPTED BY THE IN VITRO ASSEMBLY OF GOLD-LABELED CYTOSKELETAL COMPONENTS, FOLLOWED BY SILVER ENHANCEMENT.Small Business Information at Submission:Principal Investigator:
Richard D Powell
5164448815Business Contact:
Nanoprobes Inc.
25 E Loop Rd Stony Brook, NY 11790EIN/Tax ID:DUNS: N/ANumber of Employees: N/AWoman-Owned: NoMinority-Owned: NoHUBZone-Owned: No
Award Information
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Agency: National Science Foundation |
Branch: N/A |
Award ID: 27402 |
Program Year/Program: 1994 / SBIR |
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Agency Tracking Number: 27402 |
Solicitation Year: N/A |
Solicitation Topic Code: N/A |
Solicitation Number: N/A |