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MOLECULAR CYTOSKELETAL MICROSCOPY PROBES AND BIOLOGICAL WIRES

Award Information

Agency:
National Science Foundation
Branch:
N/A
Award ID:
27402
Program Year/Program:
1994 / SBIR
Agency Tracking Number:
27402
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
NANOPROBES, INC.
95 HORSEBLOCK RD YAPHANK, NY 11980-
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 1994
Title: MOLECULAR CYTOSKELETAL MICROSCOPY PROBES AND BIOLOGICAL WIRES
Agency: NSF
Contract: N/A
Award Amount: $64,993.00
 

Abstract:

NOVEL PROBES ARE INTRODUCED FOR THE STUDY OF CYTOSKELETAL STRUCTURES AND PROCESSES BY ELECTRON MICROSCOPY. THESE COMPRISE LARGE CLUSTER COMPLEXES OF GOLD, PLANTINUM, AND PALLADIUM 0.8 NM-2.4 NM IN DIAMETER COVALENTLY LINKED TO PHALLOIDIN, CYTOCHALASIN B, COLCHICINE, AND TAXOL VIA A SINGLE REACTIVE FUNCTIONAL GROUP INCORPORATED INTO THEIR LIGAND SPHERES. THE CONJUGATES CAN PENETRATE 20-30 MUM OR MORE INTO CELL SECTIONS AND ACHIEVE SIGNIFICANTLY HIGHER RESOLUTION THAN COLLOIDAL GOLD-ANTIBODY CONJUGATES. THEY ARE MICROINJECTABLE; COMPATIBLE WITH LIVING CELLS; AND USED TO EXTEND THE USE OF ELECTRON MICROSCOPY TO THE STUDY OF ORGANISMS SUCH AS PROCARYOTIC BACTERIA, IN WHICH ANTIBODY PROBES ARE NOT FEASIBLE. COMBINED LABELS INCLUDING BOTH A GOLD CLUSTER AND A FLUORESCENT MOIETY ARE BEING USED TO PREPARE ADDITIONAL PROBES WITH THE SAME MOLECULES, ENABLING CORRELATION OF FLUORESCENCE CONFOCAL MICROSCOPY AND ELECTRON MICROSCOPY DATA FROM A SINGLE LABELING EXPERIMENT. THE PREPARATION OF MOLECULAR WIRES IS BEING ATTEMPTED BY THE IN VITRO ASSEMBLY OF GOLD-LABELED CYTOSKELETAL COMPONENTS, FOLLOWED BY SILVER ENHANCEMENT.

Principal Investigator:

Richard D Powell
5164448815

Business Contact:

Small Business Information at Submission:

Nanoprobes Inc.
25 E Loop Rd Stony Brook, NY 11790

EIN/Tax ID:
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No