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LIVE CELL CORRELATIVE IMAGING PROBES

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
60221
Program Year/Program:
2002 / SBIR
Agency Tracking Number:
HG002623
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
NANOPROBES, INC.
95 HORSEBLOCK RD YAPHANK, NY 11980-
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2002
Title: LIVE CELL CORRELATIVE IMAGING PROBES
Agency: HHS
Contract: 1R43HG002623-01
Award Amount: $129,956.00
 

Abstract:

DESCRIPTION (provided by applicant):Probes will be prepared, based on the 1.4 nm Nanogold cluster label, for the correlative fluorescence and electron microscopy of living cells, which may be transfected without requiring physical or chemical disruption of the cell membrane for transfection. Three approaches will be investigated: preparation of membrane permeable analogs of combined fluorescent and Nanogold antibody probes either by the incorporation of permeabilizing chemical groups such as acetoxymethyl, or by the conjugation of peptides corresponding to protein transduction domains such as the HIV-1 Tat peptide to facilitate delivery through cell membranes; preparation of membrane permeable gold cluster probes targeted to green and other colored fluorescent proteins by means of antibodies or nitrilotriacetic acid groups which target fused polyhistidine tags; and conjugates of Nanogold with FlAsH or with its corresponding binding peptide targeted respectively to the expressed peptide or to FlAsH-labeled targets. The new probes will be evaluated in the correlative localization of genes, RNA and expressed proteins in the mammalian cell nucleus, and to study the large-scale chromatin structure in the nucleus.

Principal Investigator:

Richard D. Powell
9198456324
RPOWELL@NANOPROBES.COM

Business Contact:

James Hainfeld
6312059490
HAINFELD@NANOPROBES.COM
Small Business Information at Submission:

NANOPROBES, INC.
NANOPROBES, INC. 95 HORSEBLOCK RD YAPHANK, NY 11980

EIN/Tax ID: 113038451
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No