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Application of plant genomics to alcoholic brain damage

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
71109
Program Year/Program:
2004 / STTR
Agency Tracking Number:
AA014555
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
NAPROGENIX, INC
KTRDC - U.K. - AgTecc Laboratories Lexington, KY 40546-0236
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Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2004
Title: Application of plant genomics to alcoholic brain damage
Agency: HHS
Contract: 1R41AA014555-01
Award Amount: $97,370.00
 

Abstract:

DESCRIPTION (provided by applicant): Alcoholic liver damage (ALD) is among the ten most common causes of death in the USA. The liver may be uniquely sensitive because it metabolizes ethanol at a high rate. This generates acetaldehyde intracellularly, damaging cell components directly, and/or inducing apoptotic cell death. Although some plant natural products are protective it would be of value to discover agents with greater efficacy against ALD. Here, a novel biotechnology, based on selection of mutant plant cells for resistance to alcohol, followed by high throughput pharmacological screening (HTPS) of extracts from surviving cells, is applied to this problem. The mutant population will be generated by activation tagging mutagensis, and extracts from the resulting alcohol-resistant clonal cultures screened for protection of hepatocyte cultures against ethanol-induced damage. However, because plant cells in culture metabolize ethanol to a very limited extent, we propose first to produce transgenic plant lines (N tabacum) over-expressing the activated gene for plant or mammalian ADH. Cell cultures derived from these plants will be evaluated for similarities to mammalian liver cells in metabolism of ethanol to acetaldehyde. Phase 1 is the creation of these ADH-expressing (ADHE) lines. In subsequent phases, hepatoprotective compounds from resitant clonal extracts will be tentatively identified by GC/MS. Regardless of whether novel compounds are discovered, the ADHE plant lines, and the ethanol resistant clonal cultures are patentable and represent a valuable resource for development of alcohol-related medications in the future.

Principal Investigator:

John M. Littleton
8592571085
JLITTLET@UKY.EDU

Business Contact:


8592930320
Small Business Information at Submission:

NAPROGENIX, INC.
NAPROGENIX, INC. NAPROGENIX CORPORATE OFFICES LEXINGTON, KY 40509

EIN/Tax ID: 571144999
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
Research Institution Information:
UNIVERSITY OF KENTUCKY
UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION
LEXINGTON, KY 40506
RI Type: Nonprofit college or university