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HIGH-THROUGHPUT DNA MICROARRAYS FOR INFLAMMATION

Award Information

Agency:
Department of Health and Human Services
Branch:
N/A
Award ID:
60493
Program Year/Program:
2002 / SBIR
Agency Tracking Number:
AI051921
Solicitation Year:
N/A
Solicitation Topic Code:
N/A
Solicitation Number:
N/A
Small Business Information
NUVOGEN RESEARCH, LLC
PO Box 64326 Tucson, AZ 85728-0000
View profile »
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No
 
Phase 1
Fiscal Year: 2002
Title: HIGH-THROUGHPUT DNA MICROARRAYS FOR INFLAMMATION
Agency: HHS
Contract: 1R43AI051921-01
Award Amount: $592,698.00
 

Abstract:

A quantitative high-throughput gene chip assay, ArrayPlateTM, based on nuclease protection has been developed for drug discovery. We will use this technology to develop two novel assays designed to screen for compounds that affect the expression of transcription factors and regulated genes involved in inflammation. Small changes in expression for any of the genes will be quantified for cells exposed to putative drug candidates. One assay will quantify two specific transcription factors involved in inflammation and several genes they regulate (16 total). Another will test expression of a wide panel of transcription factors implicated in the immune response alongside factors controlling other physiological pathways (300 total). A model library of 2000 novel steroidal compounds will be screened. ArrayPlateTM allows DNA array technology to be used in high throughput - with accuracy and precision. Cells are lysed in the presence of nuclease protection oligonucleotides, which hybridize to and protect all targeted mRNAs. After nuclease digestion oligonucleotides bound stochiometrically to targeted sequences are quantified on gene chips. Hybridization specificity is confirmed within each sample. The method is sensitive enough to measure very rare mRNAs - down to one mRNA molecule per cell, from 30,000 cells grown in a 96-well plate.

Principal Investigator:

Stephen Felder
5209061998
STEVEFELDER@EARTHLINK.NET

Business Contact:

Richard Kris
5209062002
RICHARDKRIS@EARTHLINK.NET
Small Business Information at Submission:

NEOGEN, LLC
NEOGEN, LLC BOX 64326, UNIVERSITY OF ARIZONA TUCSON, AZ 85728

EIN/Tax ID: 860897386
DUNS: N/A
Number of Employees: N/A
Woman-Owned: No
Minority-Owned: No
HUBZone-Owned: No